Show simple item record

dc.contributor.authorSilva, Evelyn
dc.contributor.authorValdez, Jorge
dc.contributor.authorHolmes, David
dc.contributor.authorShmaryahu, Amir
dc.contributor.authorValenzuela, Pablo
dc.date.accessioned2014-06-03T15:06:50Z
dc.date.accessioned2016-05-24T18:42:29Z
dc.date.available2014-06-03T15:06:50Z
dc.date.available2016-05-24T18:42:29Z
dc.date.issued2006
dc.identifier.citationBiol. Res. v.39 n.2 Santiago 2006es
dc.identifier.issn0716-9760
dc.identifier.otherhttp://dx.doi.org/10.4067/S0716-97602006000200018
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/2467
dc.descriptionhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200018&lng=es&nrm=isoes
dc.description.abstractBotrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11,482 expressed sequence tags that were assembled into 1,003 contigs sequences and 3,032 singletons. Approximately 81% of the unigenes showed significant similarity to genes coding for proteins with known functions: more than 50% of the sequences code for genes involved in cellular metabolism, 12% for transport of metabolites, and approximately 10% for cellular organization. Other functional categories include responses to biotic and abiotic stimuli, cell communication, cell homeostasis, and cell development. We carried out pair-wise comparisons with fungal databases to determine the B. cinerea unisequence set with relevant similarity to genes in other fungal pathogenic counterparts. Among the 4,035 non-redundant B. cinerea unigenes, 1,338 (23%) have significant homology with Fusarium verticillioides unigenes. Similar values were obtained for Saccharomyces cerevisiae and Aspergillus nidulans (22% and 24%, respectively). The lower percentages of homology were with Magnaporthe grisae and Neurospora crassa (13% and 19%, respectively). Several genes involved in putative and known fungal virulence and general pathogenicity were identified. The results provide important information for future research on this fungal pathogen.es
dc.language.isoenes
dc.publisherSociedad de Biología de Chilees
dc.subjectBotrytis cinereaes
dc.subjectcDNA sequencinges
dc.subjectfungal pathogenicityes
dc.titleGeneration and analysis of expressed sequence tags from Botrytis cinereaes
dc.typeArticlees


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record