Genomic organization of nucleolin gene in carp fish: Evidence for several genes
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Fecha
2006
Profesor/a Guía
Facultad/escuela
Idioma
en
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Editor
Sociedad de Biología de Chile
Nombre de Curso
Licencia CC
Licencia CC
Resumen
The protein nucleolin, functionally involved in the main steps of ribosome
biogenesis, is codified by a single copy gene in mammals. Here we report that at
least three different genes codify for this protein in carp fish (Cyprinus carpio). This
is the first description of the genomic organization of nucleolin in a teleost. The carp
nucleolin gene includes 8.8 kb and contains 16 exons. Promoter cis regulatory
elements are similar to constitutive genes, i.e., a putative TATA box, three G/C
boxes, and three pyrimidine-rich boxes. As in other species, carp nucleolin gene
introns host three snoRNA codifying sequences: U23 from the H/ACA family and two
C/D box snoRNAs, U20 and U82. Both U20 and U82 span a complementary
sequence with carp 18S rRNA. Additionally, we identified two cDNAs coding for
nucleolin, confirming the existence of several nucleolin genes in carp. Amino acidderived
sequence from carp cDNAs differ from mammal protein because they span
additional acidic domains at the amino end, whose functional significance remains
unclear. We performed amino acid sequence comparison and phylogenetic analyses
showing that the three isoforms of carp nucleolin, which we describe herein, cluster
in two groups. cNUC1 probably diverges from cNUC2 and cNUC3 as result of
ancestral fish-specific genome duplication, indeed C. carpio is a tetraploid fish.
Notas
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200017&lng=es&nrm=iso
Palabras clave
Nucleolin, Nucleolin, Nucleolus, U snoRNAs, rRNA 18S, Carp fish
Citación
Biol. Res. v.39 n.2 Santiago 2006