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Genomic organization of nucleolin gene in carp fish: Evidence for several genes

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dc.contributor.author Quezada, Claudia
dc.contributor.author Navarro, Cristina
dc.contributor.author San Martín, Rody
dc.contributor.author Alvarez, Marco
dc.contributor.author Molina, Alfredo
dc.contributor.author Vera, Ines
dc.date.accessioned 2014-06-03T15:29:54Z
dc.date.accessioned 2016-05-24T18:43:57Z
dc.date.available 2014-06-03T15:29:54Z
dc.date.available 2016-05-24T18:43:57Z
dc.date.issued 2006
dc.identifier.citation Biol. Res. v.39 n.2 Santiago 2006 es
dc.identifier.issn 0716-9760
dc.identifier.other DOI: http://dx.doi.org/10.4067/S0716-97602006000200017
dc.identifier.uri http://repositorio.unab.cl/xmlui/handle/ria/2468
dc.description http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200017&lng=es&nrm=iso es
dc.description.abstract The protein nucleolin, functionally involved in the main steps of ribosome biogenesis, is codified by a single copy gene in mammals. Here we report that at least three different genes codify for this protein in carp fish (Cyprinus carpio). This is the first description of the genomic organization of nucleolin in a teleost. The carp nucleolin gene includes 8.8 kb and contains 16 exons. Promoter cis regulatory elements are similar to constitutive genes, i.e., a putative TATA box, three G/C boxes, and three pyrimidine-rich boxes. As in other species, carp nucleolin gene introns host three snoRNA codifying sequences: U23 from the H/ACA family and two C/D box snoRNAs, U20 and U82. Both U20 and U82 span a complementary sequence with carp 18S rRNA. Additionally, we identified two cDNAs coding for nucleolin, confirming the existence of several nucleolin genes in carp. Amino acidderived sequence from carp cDNAs differ from mammal protein because they span additional acidic domains at the amino end, whose functional significance remains unclear. We performed amino acid sequence comparison and phylogenetic analyses showing that the three isoforms of carp nucleolin, which we describe herein, cluster in two groups. cNUC1 probably diverges from cNUC2 and cNUC3 as result of ancestral fish-specific genome duplication, indeed C. carpio is a tetraploid fish. es
dc.language.iso en es
dc.publisher Sociedad de Biología de Chile es
dc.subject Nucleolin es
dc.subject Nucleolin es
dc.subject Nucleolus es
dc.subject U snoRNAs es
dc.subject rRNA 18S es
dc.subject Carp fish es
dc.title Genomic organization of nucleolin gene in carp fish: Evidence for several genes es
dc.type Article es


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