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dc.contributor.authorQuezada, Claudia
dc.contributor.authorNavarro, Cristina
dc.contributor.authorSan Martín, Rody
dc.contributor.authorAlvarez, Marco
dc.contributor.authorMolina, Alfredo
dc.contributor.authorVera, Ines
dc.date.accessioned2014-06-03T15:29:54Z
dc.date.accessioned2016-05-24T18:43:57Z
dc.date.available2014-06-03T15:29:54Z
dc.date.available2016-05-24T18:43:57Z
dc.date.issued2006
dc.identifier.citationBiol. Res. v.39 n.2 Santiago 2006es
dc.identifier.issn0716-9760
dc.identifier.otherDOI: http://dx.doi.org/10.4067/S0716-97602006000200017
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/2468
dc.descriptionhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200017&lng=es&nrm=isoes
dc.description.abstractThe protein nucleolin, functionally involved in the main steps of ribosome biogenesis, is codified by a single copy gene in mammals. Here we report that at least three different genes codify for this protein in carp fish (Cyprinus carpio). This is the first description of the genomic organization of nucleolin in a teleost. The carp nucleolin gene includes 8.8 kb and contains 16 exons. Promoter cis regulatory elements are similar to constitutive genes, i.e., a putative TATA box, three G/C boxes, and three pyrimidine-rich boxes. As in other species, carp nucleolin gene introns host three snoRNA codifying sequences: U23 from the H/ACA family and two C/D box snoRNAs, U20 and U82. Both U20 and U82 span a complementary sequence with carp 18S rRNA. Additionally, we identified two cDNAs coding for nucleolin, confirming the existence of several nucleolin genes in carp. Amino acidderived sequence from carp cDNAs differ from mammal protein because they span additional acidic domains at the amino end, whose functional significance remains unclear. We performed amino acid sequence comparison and phylogenetic analyses showing that the three isoforms of carp nucleolin, which we describe herein, cluster in two groups. cNUC1 probably diverges from cNUC2 and cNUC3 as result of ancestral fish-specific genome duplication, indeed C. carpio is a tetraploid fish.es
dc.language.isoenes
dc.publisherSociedad de Biología de Chilees
dc.subjectNucleolines
dc.subjectNucleolines
dc.subjectNucleoluses
dc.subjectU snoRNAses
dc.subjectrRNA 18Ses
dc.subjectCarp fishes
dc.titleGenomic organization of nucleolin gene in carp fish: Evidence for several geneses
dc.typeArticlees


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