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A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses

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dc.contributor.author Meissel, Lee
dc.contributor.author Fonseca, Beatriz
dc.contributor.author Gonzalez, Susana
dc.contributor.author Baeza-Yates, Ricardo
dc.contributor.author Cambiazo, Veronica
dc.contributor.author Campos, Reinaldo
dc.contributor.author Gonzalez, Mauricio
dc.contributor.author Orellana, Ariel
dc.contributor.author Retamales, Julio
dc.contributor.author Silva, Herman
dc.date.accessioned 2014-07-02T20:08:30Z
dc.date.accessioned 2016-05-24T18:07:57Z
dc.date.available 2014-07-02T20:08:30Z
dc.date.available 2016-05-24T18:07:57Z
dc.date.issued 2005
dc.identifier.citation Biol. Res. v.38 n.1 Santiago 2005 es
dc.identifier.issn 0716-9760
dc.identifier.uri http://repositorio.unab.cl/xmlui/handle/ria/2476
dc.description http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010&lng=es&nrm=iso es
dc.description.abstract Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the fruits of Prunus persica. This RNA may be used for many functional genomic based experiments such as RT-PCR and the construction of large-insert cDNA libraries. es
dc.language.iso en es
dc.publisher Sociedad de Biología de Chile es
dc.subject cDNA library es
dc.subject fruit es
dc.subject fruit trees es
dc.subject functional genomics es
dc.subject peach es
dc.subject polysaccharides es
dc.subject Prunus persica es
dc.subject RNA isolation es
dc.subject Rosaceae es
dc.subject RT-PCR es
dc.title A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses es
dc.type Article es

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