Complete sequence of the genome of the human isolate of Andes virus CHI-7913: comparative sequence and protein structure analysis
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Fecha
2003
Profesor/a Guía
Facultad/escuela
Idioma
en
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Editor
Sociedad de Biología de Chile
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Licencia CC
Licencia CC
Resumen
We report here the complete genomic sequence of the Chilean human isolate of Andes virus CHI-7913. The S, M, and L genome segment sequences of this isolate are 1,802, 3,641 and 6,466 bases in length, with an overall GC content of 38.7%. These genome segments code for a nucleocapsid protein of 428 amino acids, a glycoprotein precursor protein of 1,138 amino acids and a RNA-dependent RNA polymerase of 2,152 amino acids. In addition, the genome also has other ORFs coding for putative proteins of 34 to 103 amino acids. The encoded proteins have greater than 98% overall similarity with the proteins of Andes virus isolates AH-1 and Chile R123. Among other sequenced Hantavirus, CHI-7913 is more closely related to Sin Nombre virus, with an overall protein similarity of 92%. The characteristics of the encoded proteins of this isolate, such as hydrophobic domains, glycosylation sites, and conserved amino acid motifs shared with other Hantavirus and other members of the Bunyaviridae family, are identified and discussed.
Notas
Indexación: Scielo
Palabras clave
Hantavirus, genome sequence, RNA polymerase, human isolate
Citación
Biological Research, Vol.36 N° 2. Santiago, 2003.