The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development
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Fecha
2016-07
Profesor/a Guía
Facultad/escuela
Idioma
en
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Editor
NATURE PUBLISHING GROUP
Nombre de Curso
Licencia CC
Licencia CC
Resumen
Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.
Notas
Indexación: Web of Science.
Palabras clave
DE-NOVO SYNTHESIS, GALACTOSE TRANSPORTER, RHAMNOGALACTURONAN-II, SUGAR TRANSPORTERS, N-GLYCANS, UDP-GALACTOSE, MUR1 GENE, THALIANA, XYLOGLUCAN, EXPRESSION
Citación
Nature Communications 7, Article number:12119 (2016)