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SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY

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dc.contributor.author ARAYA, EYLEEN
dc.contributor.author KOGAN, MARCELO J.
dc.contributor.author GUELL, ALEIX G.
dc.contributor.author ESCOBAR, CARLOS A.
dc.contributor.author SANZ, FAUSTO
dc.date.accessioned 2016-07-04T15:44:16Z
dc.date.available 2016-07-04T15:44:16Z
dc.date.issued 2014-07
dc.identifier.citation J. Chil. Chem. Soc. vol.59 no.2 Concepción July 2014 es
dc.identifier.issn 0717-9707
dc.identifier.other http://dx.doi.org/10.4067/S0717-97072014000200013
dc.identifier.uri http://repositorio.unab.cl/xmlui/handle/ria/943
dc.description Indexación: Web of Science; Scielo. es
dc.description.abstract A hydrogen-terminated Si (111) surface was modified to form an aminoterminated monolayer for immobilization of streptavidin. Cleavage of an N-(ω-undecylenyl)-phthalimide covered surface using hidrazine yields an amino group-modified surface, which serves as a substrate for the attachment of biotin and subsequently streptavidin. We used surface analytical techniques to characterize the surface and to control the course of functionalization before the immobilization of streptavidin. To confirm the presence of the streptavidin Texas red on the surface two powerful techniques available in a standard biochemical laboratory are used, Fluorescence Microscopy and MALDI-TOF that allow us to detect and determine the immobilized streptavidin. This work provides an avenue for the development of devices in which the exquisite binding specificity of biomolecular recognition is directly coupled to a biosensor. In addition, we have demonstrated that MALDI-TOF and fluorescence microscopy are useful techniques for the characterization of silicon functionalized surfaces. es
dc.description.uri http://ref.scielo.org/gm87cv
dc.language.iso en es
dc.publisher Sociedad Chilena de Química es
dc.subject Química es
dc.title SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY es
dc.type Article es


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