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Caries-free subjects have high levels of urease and arginine deiminase activity

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dc.contributor.author Reyes, Evelyn
dc.contributor.author Martin, Javier
dc.contributor.author Moncada, Gustavo
dc.contributor.author Neira, Miguel
dc.contributor.author Palma, Patricia
dc.contributor.author Gordan, Valeria
dc.contributor.author Oyarzo, Juan Fernando
dc.contributor.author Yévenes, Ismael
dc.date.accessioned 2016-07-04T16:22:24Z
dc.date.available 2016-07-04T16:22:24Z
dc.date.issued 2014
dc.identifier.citation J. Appl. Oral Sci. vol.22 no.3 Bauru May./June 2014 es
dc.identifier.issn 1678-7757
dc.identifier.other http://dx.doi.org/10.1590/1678-775720130591
dc.identifier.uri http://repositorio.unab.cl/xmlui/handle/ria/947
dc.description Indexación: Web of Science; Scielo. es
dc.description.abstract Objectives: This study investigated the relationship between urease and arginine deiminase system (ADS) activities and dental caries through a cross-sectional study. Material and Methods: Urease and ADS activities were measured in saliva and plaque samples from 10 caries-free subjects and 13 caries-active. Urease activity was obtained from the ammonia produced by incubation of plaque and saliva samples in urea. ADS activity was obtained from the ammonia generated by the arginine-HCl and Tris-maleate buffer. Specific activity was defined as micromoles of ammonia per minute per milligram of protein. Shapiro-Wilk statistical test was used to analyze the distribution of the data, and Mann-Whitney test was used to determine the significance of the data. Results: The specific urease activity in saliva and plaque was significantly higher in individuals with low DMFT scores. ADS activity in saliva (6.050 vs 1.350, p=0.0154) and plaque (8.830 vs 1.210, p=0.025) was also higher in individuals with low DMFT scores. Conclusions: Caries-free subjects had a higher ammonia generation activity by urease and arginine deiminase system for both saliva and plaque samples than low caries-active subjects. High levels of alkali production in oral environment were related to caries-free subjects. es
dc.description.uri http://ref.scielo.org/ch4qbr
dc.language.iso en es
dc.publisher Faculdade De Odontologia De Bauru - USP es
dc.subject Alkali es
dc.subject Arginine es
dc.subject Dental plaque es
dc.subject Saliva es
dc.subject Urease es
dc.subject Dental caries es
dc.title Caries-free subjects have high levels of urease and arginine deiminase activity es
dc.type Article es

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