Isolation and characterization of homokaryotic strains from the ligninolytic basidiomycete Ceriporiopsis subvermispora
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Fecha
2001-05
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
Federation of European Microbiological Societies
Nombre de Curso
Licencia CC
Licencia CC
Resumen
Genetic analyses of the lignin-degrading fungus Ceriporiopsis subvermispora is complicated by a dikaryotic nuclear condition and the absence of spore forms. Previous investigations had identified a family of closely related sequences encoding manganese peroxidase (MnP), but the relationship between genes and allelic variants could not be experimentally established. Addressing this issue, homokaryotic derivatives of C. subvermipora strain FP105752 were isolated from regenerated protoplasts. Designated CsA and CsB, their homokaryotic nature was established by polymerase chain reaction amplification and sequence analysis of the allelic variants of three MnP genes. Isoelectrofocusing revealed fewer MnP isoenzymes in filtrates of homokaryon cultures relative to the parental strain. The homokaryotic strains will simplify genetic analyses, particularly the identification of new genes.
Notas
Indexación: Scopus.
Palabras clave
Basidiomycete, Ceriporiopsis subvermispora, Homokaryon, Ligninolytic, Manganese peroxidase, Amino Acid Sequence, Base Sequence, Basidiomycota, DNA, Fungal, Isoelectric Focusing, Karyotyping, Lignin, Molecular Sequence Data, Peroxidases, Polymerase Chain Reaction, Protoplasts, Fungi
Citación
FEMS Microbiology Letters Volume 199, Issue 1, 15 May 2001, Pages 91-96
DOI
DOI: 10.1016/S0378-1097(01)00156-2