Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions
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Fecha
2013
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Facultad/escuela
Idioma
en
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Pontificia Universidad Católica de Valparaíso, Facultad de Recursos Naturales, Escuela de Ciencias del Mar
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Licencia CC
Licencia CC
Resumen
RESUMEN. En Zonas Costeras rocosas, la desecación es gatillada porción Cambios Diarios en los Niveles de marea, y la Evidencia indica experimental de Me Distribución de las algas en la zona intermareal no está Relacionada estafa do palabra capacidad, párr tolerar la desecación. En Este Contexto, la Presencia de Pyropia columbina en la zona alta del intermareal sí Explica Por Su excepcional tolerancia fisiológica a la desecación. Este Estudio explora las Vías Metabólicas involucradas en la tolerancia a la desecación en P. columbina, un Través de la Caracterización de do transcriptoma Bajo Condiciones de hidratación contrastantes. Se obtuvó 1410 TER provenientes de dos Librerías de substracción de ADNc de frondas Naturalmente hidratadas y desecadas. Los transcriptomas de emba Librerías contienen transcritos de Diversas Rutas Metabólicas Relacionadas a la tolerancia. Entre el los transcritos expresados 15% estan involucrados en la Síntesis de Proteínas, do Procesamiento y degradacion, 14,4% Asociados un Fotosíntesis y cloroplasto, el 13,1% una mitocondrial Respiración and function, 10,6% al metabolism de la Pared Celular y 7,5% a la Actividad ANTIOXIDANTE, Proteínas chaperonas y factors de Defensa (catalasa, tiorredoxina, Proteínas de choque térmico, P450 citocromo). In Ambás Librerías sí DESTACA La Presencia De genes / Proteínas no descritos en algas. Proporciona Información This El Primer Trabajo molecular Que Estudia la tolerancia a desecación en P. columbina y Sus Resultados Ayudan a explicar los patrones clásicos de Distribución descritos párr algas en la zona intermareal.
Palabras clave: Pyropia, desecación porción Estrés, EST, macroalgas, transcriptómica, Proteínas.
ABSTRACT. In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15% appears involved in protein synthesis, processing and degradation, 14.4% are related to photosynthesis and chloroplast, 13.1% to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6% to cell wall metabolism, and 7.5% are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone.
ABSTRACT. In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15% appears involved in protein synthesis, processing and degradation, 14.4% are related to photosynthesis and chloroplast, 13.1% to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6% to cell wall metabolism, and 7.5% are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone.
Notas
Indexación: Web of Science; Scielo.
Palabras clave
Pyropia, estrés desecación, las tecnologías ecológicamente racionales, las algas marinas, transcriptómica, proteínas., Pyropia, desecación porción Estrés, EST, macroalgas, transcriptómica, Proteínas.
Citación
Latin American Journal of Aquatic Research, Vol. 41, n° 5, pp. 933-958, 2013.