Molecular determinants for cyclo-oligosaccharide-based nanoparticle-mediated effective siRNA transfection
No hay miniatura disponible
Archivos
Fecha
2017-07
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
Future Medicine Ltd.
Nombre de Curso
Licencia CC
CC BY-NC-ND 4.0 DEED
Atribución-NoComercial-SinDerivadas 4.0 Internacional
Licencia CC
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Resumen
Aim: To study the structural requirements that a cyclooligosaccharide-based nanoparticle must fulfill to be an efficient siRNA transfection vector. Materials & methods: siRNA protection from degradation by RNAses, transfection efficiency and the thermodynamic parameters of the nanoparticle/siRNA interactions were studied on pairs of amphiphilic molecules using biochemical techniques and molecular dynamics. Results: The lower the siRNA solvent accessible surface area in the presence of the nanoparticle, higher the protection from RNAse-mediated degradation in the corresponding nanocomplex; a moderate nanoparticle/siRNA binding energy value further facilitates reversible complexation and binding to the target cellular mRNA. Conclusion: The use, in advance, of these parameters will provide a useful indication of the potential of a molecular nanoparticle as siRNA transfecting vector. © 2017 Future Medicine Ltd.
Notas
Indexación: Scopus
Palabras clave
endosomal escape, molecular modeling, molecular nanoparticle, protection from RNAses, protein knock down, siRNA transfection
Citación
Nanomedicine Volume 12, Issue 13, Pages 1607 - 1621July 2017
DOI
10.2217/nnm-2017-0123