Regulation of a novel Acidithiobacillus caldus gene cluster involved in metabolism of reduced inorganic sulfur compounds
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Archivos
Fecha
0027-11-01
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
American Society for Microbiology
Nombre de Curso
Licencia CC
Licencia CC
Resumen
Acidithiobacillus caldus has been proposed to play a role in the oxidation of reduced inorganic sulfur compounds (RISCs) produced in industrial biomining of sulfidic minerals. Here, we describe the regulation of a new cluster containing the gene encoding tetrathionate hydrolase (tetH), a key enzyme in the RISC metabolism of this bacterium. The cluster contains five cotranscribed genes, ISac1, rsrR, rsrS, tetH, and doxD, coding for a transposase, a two-component response regulator (RsrR and RsrS), tetrathionate hydrolase, and DoxD, respectively. As shown by quantitative PCR, rsrR, tetH, and doxD are upregulated to different degrees in the presence of tetrathionate. Western blot analysis also indicates upregulation of TetH in the presence of tetrathionate, thiosulfate, and pyrite. The tetH cluster is predicted to have two promoters, both of which are functional in Escherichia coli and one of which was mapped by primer extension. A pyrrolo-quinoline quinone binding domain in TetH was predicted by bioinformatic analysis, and the presence of an o-quinone moiety was experimentally verified, suggesting a mechanism for tetrathionate oxidation.
Notas
INDEXACIÓN: WEB OF SCIENCE.
Palabras clave
ACID-MINE DRAINAGE, THIOBACILLUS-FERROOXIDANS, FERROBACILLUS-FERROOXIDANS, TETRATHIONATE HYDROLASE, QUINONE OXIDOREDUCTASEOXIDIZING BACTERIAESCHERICHIA-COLISULFIDE MINERALSOXIDATIONTHIOSULFATE, OXIDIZING BACTERIA, ESCHERICHIA-COLI, SULFIDE MINERALS, OXIDATION, THIOSULFATE
Citación
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Volume 73, Issue 22, Page 7367-7372
DOI
10.1128/AEM.01497-07