Visible light-activated mesoporous black titania nanorods for enhanced chemo-photodynamic cancer therapy
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Fecha
2025-04
Profesor/a Guía
Facultad/escuela
Idioma
en_US
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Editor
Editions de Sante
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Licencia CC
Licencia CC
Resumen
Developing photoactive nanomaterials with drug-encapsulating properties is a relevant task for dual therapy applications aimed at overcoming the current limitations of conventional cancer treatments. In this work, we report the synthesis and evaluation of mesoporous black titania nanorods as novel visible-light responsive platforms for dual chemo-phototherapy. Nanorods were obtained by hydrothermal synthesis and photo-sensitized by titanium reduction via thermal decomposition of sodium borohydride at 400 °C under an argon atmosphere. The materials were characterized by X-ray diffraction, transmission electron microscopy, N2 adsorption-desorption isotherms, X-ray photoelectron spectroscopy, electron paramagnetic resonance, and UV–vis diffuse reflectance spectroscopy. ROS generation experiments confirmed that aqueous dispersions of black titania nanorods produce hydroxyl radicals (HO•) under visible light LED irradiation (150 W m−2, 15 min). The materials exhibited moderate phototoxic activity against HeLa cells under visible light LED irradiation (150 W m−2, 15 min) with cell viabilities ∼70 % at dispersion concentrations of 300 μg mL−1, while no cytotoxic effects were observed in dark incubation conditions. Black titania nanorods efficiently encapsulated doxorubicin and released the drug in quantities suitable to exert chemotoxic effects against HeLa cells in the dark, with cell viabilities of ∼60 % at 500 μg mL−1 dispersion concentrations and 24 h of incubation. Doxorubicin-loaded nanorods were evaluated in dual chemo-phototherapy experiments consisting of 12 h of dark incubation, 15 min of visible light LED irradiation, and 12 h of post-irradiation dark incubation. Our experiments showed a marked potentiation of antiproliferative effects compared with individual chemo or phototoxicity experiments, with cell viabilities ∼30 % at dispersion concentrations of 400 μg mL−1. Our findings highlight the potential of black titania nanorods as intrinsically photoactive materials with drug-encapsulating properties for dual chemo-phototherapy applications.
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Indexación (Scopus)
Palabras clave
Chemo-photodynamic therapy, Mesoporous black titania, Visible light ROS generation
Citación
Journal of Drug Delivery Science and Technology Volume 106 April 2025 Article number 106713
DOI
10.1016/j.jddst.2025.106713