Pdgf1aa regulates zebrafish neural crest cells migration through Hif-1 in an oxygen-independent manner
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Fecha
2018-12
Autores
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
Elsevier Ireland Ltd
Nombre de Curso
Licencia CC
Licencia CC
Resumen
The transcription factor Hif-1α regulates epithelial to mesenchymal transition and neural crest cell chemotaxis in Xenopus. Hif-1α is only stabilised under low oxygen levels, and the in vivo stabilisation of this factor in neural crest cells is poorly understood. Multiple oxygen-independent Hif-1α regulators have been described in cell cultures and cancer models. Among these, the PDGF pathway has been linked to neural crest development. The present study established a connection between the Pdgf pathway and Hif-1α stabilisation in zebrafish. Specifically, embryos with a disrupted Pdgf pathway were rescued by employing hif-1α mRNA through qPCR and immunohistochemistry techniques. The data suggest that oxygen levels in the neural crest are normal and that Pdgf1aa regulates neural crest migration through Hif-1α expression. © 2018 Elsevier B.V.
Notas
Indexación Scopus
Palabras clave
Pericardium, Regeneration, Zebrafish
Citación
Mechanisms of Development Volume 154, Pages 203 - 207December 2018
DOI
10.1016/j.mod.2018.07.007