Resistance-nodulation-division efflux pump acrAB is modulated by florfenicol and contributes to drug resistance in the fish pathogen Piscirickettsia salmonis
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Fecha
2016-06
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
Oxford University Press
Nombre de Curso
Licencia CC
Licencia CC
Resumen
Piscirickettsia salmonis is a fastidious intracellular pathogen responsible for high mortality rates in farmed salmonids,
with serious economic consequences for the Chilean aquaculture industry. Oxytetracycline and florfenicol are the most
frequently used antibiotics against P. salmonis, but routine use could contribute to drug resistance. This study identified
differentiated florfenicol susceptibilities in two P. salmonis strains, LF-89 and AUSTRAL-005. The less susceptible isolate,
AUSTRAL-005, also showed a high ethidium bromide efflux rate, indicating a higher activity of general efflux pump
genes than LF-89. The P. salmonis genome presented resistance nodulation division (RND) family members, a family
containing typical multidrug resistance-related efflux pumps in Gram-negative bacteria. Additionally, efflux pump acrAB
genes were overexpressed in AUSTRAL-005 following exposure to the tolerated maximal concentration of florfenicol, in
contrast to LF-89. These results indicate that tolerated maximum concentrations of florfenicol can modulate RND gene
expression and increase efflux pump activity. We propose that the acrAB efflux pump is essential for P. salmonis survival at
critical florfenicol concentrations and for the generation of antibiotic-resistant bacterial strains.
Notas
Indexación: Scopus.
Palabras clave
Piscirickettsia Salmonis, SRS, RND Efflux Pump, Florfenicol, Antibiotic Resistance
Citación
FEMS Microbiology Letters. Volume 363, Issue 11, Pages 1 - 7. 1 June 2016
DOI
10.1093/femsle/fnw102