Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings
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Archivos
Fecha
2008-05-19
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
BMC
Nombre de Curso
Licencia CC
CC BY 2.0
Attribution 2.0 Generic
Deed
Licencia CC
https://creativecommons.org/licenses/by/2.0/
Resumen
Background: The isolation of green fluorescent protein (GFP) and the development of spectral variants over the past decade have begun to reveal the dynamic nature of protein trafficking and organelle motility. In planta analyses of this dynamic process have typically been limited to only two organelles or proteins at a time in only a few cell types.
Results: We generated a transgenic Arabidopsis plant that contains four spectrally different fluorescent proteins. Nuclei, plastids, mitochondria and plasma membranes were genetically tagged with cyan, red, yellow and green fluorescent proteins, respectively. In addition, methods to track nuclei, mitochondria and chloroplasts and quantify the interaction between these organelles at a submicron resolution were developed. These analyzes revealed that N-ethylmaleimide disrupts nuclear-mitochondrial but not nuclear-plastids interactions in root epidermal cells of live Arabidopsis seedlings.
Conclusion: We developed a tool and associated methods for analyzing the complex dynamic of organelle-organelle interactions in real time in planta. Homozygous transgenic Arabidopsis (Kaleidocell) is available through Arabidopsis Biological Resource Center.
Notas
INDEXACIÓN: WEB OF SCIENCE.
Palabras clave
PLASMA-MEMBRANE, MITOCHONDRIA, ACTIN, CELL, MICROTUBULES, STROMULES, MOVEMENT, PROTEINS, DYNAMICS, PLASTIDS
Citación
PLANT METHODS, Volume 4
DOI
10.1186/1746-4811-4-9