Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase
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Archivos
Fecha
2017-12
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
Wiley-Blackwell
Nombre de Curso
Licencia CC
CC BY-NC-ND 4.0 DEED
Licencia CC
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Resumen
Novel bone regeneration approaches aim to obtain immature osteoblasts from somatic stem cells. Umbilical cord Wharton's jelly mesenchymal stem cells (WJ-MSCs) are an ideal source for cell therapy. Hence, the study of mechanisms involved in WJ-MSC osteoblastic differentiation is crucial to exploit their developmental capacity. Here, we have assessed epigenetic control of the Runt-related transcription factor 2 (RUNX2) osteogenic master regulator gene in WJ-MSC. We present evidence indicating that modulation of RUNX2 expression through preventing Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase activity is relevant to enhance WJ-MSC osteoblastic potential. Hence, JARID1B loss of function in WJ-MSC results in increased RUNX2/p57 expression. Our data highlight JARID1B activity as a novel target to modulate WJ-MSC osteoblastic differentiation with potential applications in bone tissue engineering. Stem Cells 2017;35:2430–2441. © 2017 AlphaMed Press
Notas
Indexación: Scopus
Palabras clave
Epigenetics, Mesenchymal stem cells (MSCs), Osteoblasts, Umbilical cord
Citación
Stem Cells Volume 35, Issue 12, Pages 2430 - 2441December 2017
DOI
10.1002/stem.2704