Survival of Clostridium difficile spores at low water activity

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dc.contributor.authorDeng, Kai
dc.contributor.authorTalukdar, Prabhat K.
dc.contributor.authorSarker, Mahfuzur R.
dc.contributor.authorParedes-Sabja, Daniel
dc.contributor.authorTorres, J. Antonio
dc.date.accessioned2024-04-25T14:19:04Z
dc.date.available2024-04-25T14:19:04Z
dc.date.issued2017-08
dc.descriptionIndexación: Scopus
dc.description.abstractClostridium difficile is frequently found in meat and meat products. Germination efficiency, defined as colony formation, was previously investigated at temperatures found in meat handling and processing for spores of strain M120 (animal isolate), R20291 (human isolate), and DK1 (beef isolate). In this study, germination efficiency of these spore strains was assessed in phosphate buffered saline (PBS, aw ∼1.00), commercial beef jerky (aw ∼0.82/0.72), and aw-adjusted PBS (aw ∼0.82/0.72). Surface hydrophobicity was followed for spores stored in PBS. After three months and for all PBS aw levels tested, M120 and DK1 spores showed a ∼1 decimal reduction in colony formation but this was not the case when kept in beef jerky suggesting a protective food matrix effect. During storage, and with no significant aw effect, an increase in colony formation was observed for R20291 spores kept in PBS (∼2 decimal log increase) and beef jerky (∼1 decimal log increase) suggesting a loss of spore superdormancy. For all strains, no significant changes in spore surface hydrophobicity were observed after storage. Collectively, these results indicate that depending on the germination properties of C. difficile spores and the media properties, their germination efficiency may increase or decrease during long term food storage. © 2017 Elsevier Ltd
dc.description.urihttps://www-sciencedirect-com.recursosbiblioteca.unab.cl/science/article/pii/S0740002016308115?via%3Dihub
dc.identifier.citationFood Microbiology Volume 65, Pages 274 - 2781 August 2017
dc.identifier.doi10.1016/j.fm.2017.03.013
dc.identifier.issn0740-0020
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/56413
dc.language.isoen
dc.publisherAcademic Press
dc.rights.licenseCC BY 4.0 DEED Atribución 4.0 Internacional
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectClostridium difficile
dc.subjectSpore hydrophobicity
dc.subjectSpore survival
dc.subjectWater activity
dc.titleSurvival of Clostridium difficile spores at low water activity
dc.typeArtículo
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