High prevalence and characterization of extended-spectrum ß-lactamase producing Enterobacteriaceae in Chadian hospitals

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dc.contributor.authorOuchar Mahamat, Oumar
dc.contributor.authorLounnas, Manon
dc.contributor.authorHide, Mallorie
dc.contributor.authorDumont, Yann
dc.contributor.authorTidjani, Abelsalam
dc.contributor.authorKamougam, Kadidja
dc.contributor.authorAbderrahmane, Madina
dc.contributor.authorBenavides, Julio
dc.contributor.authorSolasso, Jérôme
dc.contributor.authorBañuls, Anne-Laure
dc.contributor.authorJean-Pierre, Hélène
dc.contributor.authorCarrière, Christian
dc.contributor.authorGodreuil, Sylvain
dc.date.accessioned2023-08-07T20:25:11Z
dc.date.available2023-08-07T20:25:11Z
dc.date.issued2019-02-28
dc.descriptionIndexación Scopuses
dc.description.abstractBackground: Extended-spectrum ß-lactamase-producing Enterobacteriaceae (ESBL-PE) represent a major problem in the management of nosocomial infections. However, ESBL-PE are not systematically monitored in African countries. The aim of this study was to determine ESBL-PE prevalence in patients from three hospitals in N'Djamena, the capital city of Chad, and to characterize the genetic origin of the observed resistance. Methods: From January to March 2017, 313 non-duplicate isolates were recovered from various clinical specimens obtained from 1713 patients in the three main hospitals of N'Djamena. Bacterial species were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Susceptibility to 28 antibiotics was tested using the disk diffusion method on Müller-Hinton agar, and ESBL production was confirmed with the double-disc synergy test. The most prevalent ESBL genes associated with the observed resistance were detected using multiplex PCR followed by double-stranded DNA sequencing. Results: Among the 313 isolates, 197 belonged to the Enterobacteriaceae family. The overall ESBL-PE prevalence was 47.72% (n = 94/197), with a higher rate among inpatients compared with outpatients (54.13% vs. 34.37%). ESBL-PE prevalence was highest in older patients (≥60 years of age). E. coli was the most common ESBL-producer organism (63.8%), followed by K. pneumoniae (21.2%). ESBL-PE were mainly found in urine samples (75%). The CTX-M-1 group was dominant (96.7% of the 94 ESBL-PE isolates, CTX-M-15 enzyme), followed by the CTX-M-9 group (4.1%). 86% of resistant isolates harbored more than one ESBL-encoding gene. ESBL production was also associated with the highest levels of resistance to non-β-lactam drugs. Conclusions: The prevalence of ESBL-PE harboring resistant genes encoding ESBLs of the CTX-M-1 group was high (48%) among clinical isolates of three main hospitals in Chad, suggesting an alarming spread of ESBL-PE among patients.es
dc.identifier.doi10.1186/s12879-019-3838-1en
dc.identifier.issn1471-2334
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/52274
dc.language.isoenes
dc.publisherBMC Infectious Diseaseses
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.esen
dc.subjectChades
dc.subjectClinical sampleses
dc.subjectEnterobacteriaceaees
dc.subjectESBLes
dc.subjectPrevalencees
dc.titleHigh prevalence and characterization of extended-spectrum ß-lactamase producing Enterobacteriaceae in Chadian hospitalses
dc.typeArtículoes
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