Downregulation of the polycomb-associated methyltransferase ezh2 during maturation of hippocampal neurons is mediated by microRNAs let-7 and miR-124
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Fecha
2020-11
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
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Título del volumen
Editor
MDPI AG
Nombre de Curso
Licencia CC
Licencia CC
Resumen
Ezh2 is a catalytic subunit of the polycomb repressive complex 2 (PRC2) which mediates epigenetic gene silencing through depositing the mark histone H3 lysine 27 trimethylation (H3K27me3) at target genomic sequences. Previous studies have demonstrated that Enhancer of Zeste Homolog 2 (Ezh2) was differentially expressed during maturation of hippocampal neurons; in immature neurons, Ezh2 was abundantly expressed, whereas in mature neurons the expression Ezh2 was significantly reduced. Here, we report that Ezh2 is downregulated by microRNAs (miRs) that are expressed during the hippocampal maturation process. We show that, in mature hippocampal neurons, lethal-7 (let-7) and microRNA-124 (miR-124) are robustly expressed and can target cognate motifs at the 3′-UTR of the Ezh2 gene sequence to downregulate Ezh2 expression. Together, these data demonstrate that the PRC2 repressive activity during hippocampal maturation is controlled through a post-transcriptional mechanism that mediates Ezh2 downregulation in mature neurons. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Notas
Indexación Scopus
Palabras clave
MicroRNAs, Neurogenesis, Alzheimer's Disease
Citación
International Journal of Molecular Sciences, Volume 21, Issue 22, Pages 1 - 162 November 2020 Article number 8472
DOI
10.3390/ijms21228472