Using a ligate intestinal loop mouse model to investigate Clostridioides difficile adherence to the intestinal mucosa in aged mice
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Archivos
Fecha
2021-12
Profesor/a Guía
Facultad/escuela
Idioma
en
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ISSN de la revista
Título del volumen
Editor
Public Library of Science
Nombre de Curso
Licencia CC
CC BY 4.0 Deed Atribución 4.0 Internacional
Licencia CC
https://creativecommons.org/licenses/by/4.0/deed.es
Resumen
Interaction of Clostridioides difficile spores with the intestinal mucosa contributes to the persistence and recurrence of the infection. Advanced age is one of the main risk factors for C. difficile infection and recurrence of the disease. However, interaction of C. difficile spores with the intestinal mucosa during aging has not been evaluated. In the present work, using intestinal ligated loop technique in a mouse model, we analyzed C. difficile spore adherence and internalization to the ileum and colonic mucosa during aging. Additionally, we provide visual documentation of the critical steps of the procedure. Consequently, our data suggest that spore internalization in the ileum and colonic mucosa is higher in elderly mice rather than adults or young mice. Also, our data suggest that spore adherence to the ileum and colonic mucosa decreases with aging.
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Indexación (Scopus)
Palabras clave
Aging, Animals, Attachment Sites, Microbiological, Bacterial Adhesion, Clostridioides difficile, Clostridium Infections, Disease Models, Animal, Female, Intestinal Mucosa, Male, Mice, Mice Inbred C57BL, Microscopy, Confocal, Spores, Bacterial
Citación
PLoS ONE Volume 16, Issue 12 December December 2021 Article number e0261081
DOI
10.1371/journal.pone.0261081