Modulation of Auxin Levels in Pollen Grains Affects Stamen Development and Anther Dehiscence in Arabidopsis
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2018-09
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en
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MDPI AG
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Licencia CC
Resumen
Auxin regulates diverse aspects of flower development in plants, such as differentiation of the apical meristem, elongation of the stamen, and maturation of anthers and pollen. It is known that auxin accumulates in pollen, but little information regarding the biological relevance of auxin in this tissue at different times of development is available. In this work, we manipulated the amount of free auxin specifically in developing pollen, using transgenic Arabidopsis lines that express the bacterial indole-3-acetic acid-lysine synthetase (iaaL) gene driven by a collection of pollen-specific promoters. The iaaL gene codes for an indole-3-acetic acid-lysine synthetase that catalyzes the conversion of free auxin into inactive indole-3-acetyl-L-lysine. The transgenic lines showed several abnormalities, including the absence of short stamina, a diminished seed set, aberrant pollen tubes, and perturbations in the synchronization of anther dehiscence and stamina development. This article describes the importance of auxin accumulation in pollen and its role in stamina and anther development. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
Notas
Indexación: Scopus.
Chemicals and CAS Registry Numbers: acetic acid, 127-08-2, 127-09-3, 64-19-7, 71-50-1; beta glucuronidase, 9001-45-0; synthetase, 9031-56-5, 9031-57-6; Arabidopsis Proteins; Indoleacetic Acids; Plant Growth Regulators
Funding: This work was supported by the Fondo Nacional de Desarrollo Científico y Tecnológico (1170259) and the MIISSB Iniciativa Científica Milenio-MINECON to Francisca Blanco-Herrera, the Fondo Nacional de Desarrollo Científico y Tecnológico (1120766) to Gabriel León, and the Spanish Ministry of Economy, Industry and Competitiveness (MINECO) (BFU2014-55575-R, BFU2017-82826-R) to Stephan Pollmann.
Chemicals and CAS Registry Numbers: acetic acid, 127-08-2, 127-09-3, 64-19-7, 71-50-1; beta glucuronidase, 9001-45-0; synthetase, 9031-56-5, 9031-57-6; Arabidopsis Proteins; Indoleacetic Acids; Plant Growth Regulators
Funding: This work was supported by the Fondo Nacional de Desarrollo Científico y Tecnológico (1170259) and the MIISSB Iniciativa Científica Milenio-MINECON to Francisca Blanco-Herrera, the Fondo Nacional de Desarrollo Científico y Tecnológico (1120766) to Gabriel León, and the Spanish Ministry of Economy, Industry and Competitiveness (MINECO) (BFU2014-55575-R, BFU2017-82826-R) to Stephan Pollmann.
Palabras clave
Anther, Auxin, Dehiscence, IaaL, Pollen
Citación
International Journal of Molecular Sciences, 19(9), art. no. 2480.