Purificación y caracterización fotofísica del complejo [(phen)Re(CO)3(ethinyl-py)]+: preparando las bases para el desarrollo de aplicaciones biológicas
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2016
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es
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Universidad Andrés Bello
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Licencia CC
Licencia CC
Resumen
El Renio es un metal de transición escaso a nivel de la corteza terrestre, que se obtiene principalmente a partir de la refinación de minerales de molibdeno y es utilizado comúnmente en joyería. Entre los compuestos de este metal, los complejos de Renio (I) tricarbonilo con ligando dimina han capturado la atención de la comunidad científica y han impulsado nuevos estudios debido a sus interesantes propiedades fotofísicas; las que pueden ser modificadas cambiando la naturaleza de los ligandos orgánicos con los cuales se coordina el metal de transición.
Por otra parte, los complejos de Renio (I) han sido utilizados en el desarrollo de distintas aplicaciones biológicas, así como también en la reducción fotoquímica de CO2. Enfocados en el campo de las aplicaciones biológicas de esta familia de compuestos, nace la hipótesis de formular un nuevo complejo para evaluar su potencial aplicabilidad como marcador de imagen celular y su uso en terapia.
En este trabajo, se realizó el estudio de las propiedades fotofísicas del complejo de renio [(phen)Re(CO)3(ethinyl-py)]+ sintetizado previamente en el laboratorio de Química Bioinorgánica. Para ello, se realizó la medición del espectro de absorción y emisión del complejo, y se determinaron la absortividad molar, el tiempo de vida y el rendimiento cuántico de luminiscencia de éste. Conjuntamente, se midió la producción de oxígeno molecular singulete, el decaimiento de la luminiscencia a 1270 nm se determinó su rendimiento cuántico de generación. Estos estudios fueron realizados en distintos solventes, tales como etanol, diclorometano, dimetilformamida y buffer fosfato 5mM, 100 mM NaCl y 2 mM MgCl2. Con ello se determinó la influencia de la polaridad del medio sobre el comportamiento fotoluminiscente del complejo [(phen)Re(CO)3(ethinyl-py)]+.
Rhenium is a transition metal, rare at the Earth crust, which is mainly obtained from refining molybdenum ores and is commonly used in jewelry. Among the compounds of this metal, complexes of rhenium (I) tricarbonyl with diimine ligands have captured the attention of the scientific community and they have prompted further studies because of their interesting photophysical properties; which can be modified by changing the nature of its organic ligands coordinated to the transition metal. Moreover, complexes of rhenium (I) have been used in the development of a variety of biological applications, as well as for the photochemical reduction of CO2. Focused in the field of biological applications of this family of compounds, the hypothesis of this work is to obtain a new complex to assess their potential applicability as a marker in cellular imaging and its use in therapy. In this work, it was studied the photophysical properties of the rhenium complex [(phen)Re(CO)3(ethinyl-py)]+, previously synthesized in the laboratory of bioinorganic chemistry. To do this, the absorption and emission spectra of the complex were measured. Molar absorptivity, luminescent lifetime and luminescence quantum yield were determined. Also, the luminescence decay was measured at 1270 nm to evaluate the ability of the complex to produce of singlet molecular oxygen and generation quantum yield was obtained. These studies were performed in various solvents, such as ethanol, dichloromethane, dimethylformamide and 5 mM phosphate buffer, 100 mM NaCl and 2 mM MgCl2. Thus the influence of the polarity of the medium on the photoluminescence behavior of the complex [(phen)Re(CO)3(ethinyl-py)]+ was evaluated.
Rhenium is a transition metal, rare at the Earth crust, which is mainly obtained from refining molybdenum ores and is commonly used in jewelry. Among the compounds of this metal, complexes of rhenium (I) tricarbonyl with diimine ligands have captured the attention of the scientific community and they have prompted further studies because of their interesting photophysical properties; which can be modified by changing the nature of its organic ligands coordinated to the transition metal. Moreover, complexes of rhenium (I) have been used in the development of a variety of biological applications, as well as for the photochemical reduction of CO2. Focused in the field of biological applications of this family of compounds, the hypothesis of this work is to obtain a new complex to assess their potential applicability as a marker in cellular imaging and its use in therapy. In this work, it was studied the photophysical properties of the rhenium complex [(phen)Re(CO)3(ethinyl-py)]+, previously synthesized in the laboratory of bioinorganic chemistry. To do this, the absorption and emission spectra of the complex were measured. Molar absorptivity, luminescent lifetime and luminescence quantum yield were determined. Also, the luminescence decay was measured at 1270 nm to evaluate the ability of the complex to produce of singlet molecular oxygen and generation quantum yield was obtained. These studies were performed in various solvents, such as ethanol, dichloromethane, dimethylformamide and 5 mM phosphate buffer, 100 mM NaCl and 2 mM MgCl2. Thus the influence of the polarity of the medium on the photoluminescence behavior of the complex [(phen)Re(CO)3(ethinyl-py)]+ was evaluated.
Notas
Tesis (Licenciado en Química)
Proyecto FONDECYT Iniciación 11140401
Proyecto CONICYT 79130030
Proyecto FONDECYT Iniciación 11140401
Proyecto CONICYT 79130030
Palabras clave
Compuestos de Renio, Aplicaciones Científicas