Veloso, PabloFernández, AlejandraAstorga, JessicaGonzález Quintanilla, DavidCastro, AlfredoEscobar, AlejandroHoare, AnileiHernández, Marcela2023-09-252023-09-252022-09International Journal of Molecular Sciences, Volume 23, Issue 17, September 2022, Article number 100111661-6596https://repositorio.unab.cl/xmlui/handle/ria/53299Indexación: Scopus.Apical Lesions of Endodontic Origin (ALEO) are initiated by polymicrobial endodontic canal infection. Porphyromonas gingivalis (Pg) and Porphyromonas endodontalis (Pe) lipopolysaccharides (LPS) can induce a pro-inflammatory macrophage response through their recognition by TLR2 and TLR4. However, polarization responses induced by Pg and/or Pe LPS in macrophages are not fully understood. We aimed to characterize the polarization profiles of macrophages differentiated from THP-1 cells following Pg and/or Pe LPS stimulation from reference strain and clinical isolates. A modified LPS purification protocol was implemented and the electrophoretic LPS profiles were characterized. THP-1 human monocytes differentiated to macrophages were stimulated with Pg and Pe LPS. Polarization profiles were characterized through cell surface markers and secreted cytokines levels after 24 h of stimulation. TLR2 and TLR4 cell surfaces and transcriptional levels were determined after 24 or 2 h of LPS stimulation, respectively. LPS from Pg induced a predominant M1 profile in macrophages evidenced by changes in the expression of the surface marker CD64 and pro-inflammatory cytokine profiles, TNF-α, IL-1β, IL-6, and IL-12. Pe LPS was unable to induce a significant response. TLR2 and TLR4 expressions were neither modified by Pg or Pe LPS. Pg LPS, but not Pe LPS, induced a macrophage M1 Profile. © 2022 by the authors.enLipopolysaccharideMacrophage polarizationPorphyromonas endodontalisPorphyromonas gingivalisToll-like receptorArtículoAttribution 4.0 International (CC BY 4.0)10.3390/ijms231710011