Benavides, Julio A.Salgado-Caxito, MaríliaOpazo-Capurro, AndrésMuñoz, Paulina GonzálezPiñeiro, AnaMedina, Macarena OttoRivas, LinaMunita, JoseMunita J.Millán, Javier2024-06-252024-06-252021-05Antibiotics Volume 10, Issue 5 May 2021 Article number 5102079-6382https://repositorio.unab.cl/handle/ria/57963Indexación: Scopus.Antibiotic-resistant bacteria of critical importance for global health such as extendedspectrum beta-lactamases-producing (ESBL)-Escherichia coli have been detected in livestock, dogs, and wildlife worldwide. However, the dynamics of ESBL-E. coli between these animals remains poorly understood, particularly in small-scale farms of low and middle-income countries where contact between species can be frequent. We compared the prevalence of fecal carriage of ESBL-E. coli among 332 livestock (207 cows, 15 pigs, 60 horses, 40 sheep, 6 goats, 4 chickens), 82 dogs, and wildlife including 131 European rabbits, 30 rodents, and 12 Andean foxes sharing territory in peri-urban localities of central Chile. The prevalence was lower in livestock (3.0%) and wildlife (0.5%) compared to dogs (24%). Among 47 ESBL-E. coli isolates recovered, CTX-M-group 1 was the main ESBL genotype identified, followed by CTX-M-groups 2, 9, 8, and 25. ERIC-PCR showed no cluster of E. coli clones by either host species nor locality. To our knowledge, this is the first report of ESBL-E. coli among sheep, cattle, dogs, and rodents of Chile, confirming their fecal carriage among domestic and wild animals in small-scale farms. The high prevalence of ESBL-E. coli in dogs encourages further investigation on their role as potential reservoirs of this bacteria in agricultural settings.en-USAntimicrobial resistanceblaCTX-MChileDomestic animalsE. coliExtended-spectrum beta-lactamasesWildlifeArtículoATRIBUCIÓN 4.0 INTERNACIONAL10.3390/antibiotics10050510