Cabrera, D.Cabello-Verrugio, C.Solís, N.Martín, D.S.Cofré, C.Pizarro, M.Arab, J.P.Abrigo, J.Campos, F.Irigoyen, B.Carrasco-Avino, G.Bezares, K.Riquelme, V.Riquelme, A.Arrese, M.Barrera, F.2019-05-232019-05-232018-05International Journal of Molecular Sciences, 19(5), art. no. 13391661-6596DOI: 10.3390/ijms19051339http://repositorio.unab.cl/xmlui/handle/ria/8891Indexación: Scopus.Acknowledgments: This work was financed by the project FONDECYT #1150311 a F.B.; #11171001 to D.C.; #1150327 to M.A. and #1161646 to C.C.; Comisión Nacional de Investigación Científica y Tecnologica (grant CONICYT PIA/Basal PFB12, Basal Centre for Excellence in Science and Technology to M.A.; Millennium Institute on Immunology and Immunotherapy [P09-016-F], Programa de Cooperación Internacional ECOS-Conicyt [C16S02], UNAB-DI [741-15/N] to C.C. Open access costs were covered by FONDECYT #1150311 a F.B.Background: Somatotropic axis dysfunction associated with non-alcoholic fatty liver disease (NAFLD) has potential multisystemic detrimental effects. Here, we analysed the effects of growth hormone (GH) and insulin-like growth factor-1 (IGF-1) supplementation on liver histology, adipokine profile and muscle function in an NAFLD model. Methods: C57BL/6 mice were fed with a high fat diet (HFD) for 12 weeks and were separated into three groups treated for 4 weeks with: (1) High fat diet (HFD) (n = 10); (2) HFD + GH 9 μg/g/d (n = 10); (3) HFD + IGF-1 0.02 μg/g/d (n = 9). A control group fed a chow diet was included (n = 6). Liver histology, liver triglycerides content, serum alanine aminotransferase (ALT) activity, adiponectin and leptin serum levels, in vivo muscle strength, tetanic force and muscle fibre cross-sectional area (CSA) were measured. Results: HFD + GH and HFD + IGF-1 groups showed significantly lower ALT activity compared to HFD (p < 0.01). Liver triglyceride content in HFD + GH was decreased compared to HFD (p < 0.01). Histologic steatosis score was increased in HFD and HFD + GH group (p < 0.01), whereas HFD + IGF-1 presented no difference compared to the chow group (p = 0.3). HFD + GH group presented lower serum leptin and adiponectin levels compared to HFD. GH and IGF-1 supplementation therapy reverted HFD-induced reduction in muscle strength and CSA (sarcopenia). Conclusions: GH and IGF-1 supplementation induced significant improvement in liver steatosis, aminotransferases and sarcopenia in a diet-induced NAFLD model. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.enFatty liverGrowth hormoneIGF-1Insulin growth factor 1Somatotropic axisArtículo