Ortega, XimenaVelasquez, Juan CarlosPerez, Luz M.2014-07-022016-05-232014-07-022016-05-232005Biol. Res. v.38 n.1 Santiago 20050716-9760http://dx.doi.org/10.4067/S0716-97602005000100011http://repositorio.unab.cl/xmlui/handle/ria/2475http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100011&lng=es&nrm=isoIP3 increase and de novo synthesis of scoparone are produced in the hypersensitive response (HR) of lemon seedlings against the fungus Alternaria alternata. To elucidate whether a G-protein and/or a protein tyrosine kinase (PTK) are involved in signal transduction leading to the production of such a defensive response, we studied the HR in this plant system after treatment with G-protein activators alone and PTK inhibitors in the presence of fungal conidia. No changes in the level of IP3 were detected in response to the treatment with the G-protein activators cholera toxin or mastoparan, although the HR was observed in response to these compounds as determined by the scoparone synthesis. On the contrary, the PTK inhibitors lavendustin A and 2,5-dihidroxy methyl cinnamate (DHMC) not only prevented the IP3 changes observed in response to the fungal inoculation of lemon seedlings but also blocked the development of the HR. These results suggest that the IP3 changes observed in response to A. alternata require a PTK activity and are the result of a G-protein independent Phospholipase C activity, even though the activation of a G-protein can also lead to the development of a HR. Therefore, it appears that more than one signaling pathway may be activated for the development of HR in lemon seedlings: one involving a G-protein and the other involving a PTK-dependent PLC.enIP3synthesisG-proteinprotein activatorslemon seedlingsArtículo