Retamal, PatricioCastillo-Ruiz, MarioMora, Guido C.2024-04-092024-04-092009-04-14PLoS ONE, Volume 4, Issue 5, 14 May 2009, Article number e55511932-6203https://repositorio.unab.cl/handle/ria/55785INDEXACIÓN: SCOPUS.The MgtC is a virulence factor in Salmonella Typhimurium that is required for growth at low-Mg2+ concentrations and intramacrophage survival. This gene is codified in a conserved region of the Salmonella pathogenicity island 3 (SPI-3), and is also present in the chromosome of other Salmonella serovars. In this study we characterized the MgtC factor in S. Typhi, a human specific pathogen, by using mgtC and SPI-3 mutant strains. We found that MgtC is the most important factor codified in the SPI-3 of S. Typhi for growth in low-Mg2+ media and survival within human cells. In addition, by using reporter genes we determined that the low-Mg2+ concentration, acidic media and PhoP regulator induce mgtC expression in S. Typhi. We suggest that MgtC is the most important virulence factor codified in the SPI-3 of S. Typhi. © 2009 Retamal et al.enBacterial ProteinsCation Transport ProteinsConserved SequenceGenes, BacterialGenes, ReporterMagnesiumPhenotypeSalmonella typhiVirulence FactorsCC BY 4.0 DEED Atribución 4.0 Internacional10.1371/journal.pone.0005551