Iwanami, NorimasaSikora, KatarzynaRichter, Andreas S.Mönnich, MarenGuerri, LuciaSoza-Ried, CristianLawir, Divine-FondzenyuyMateos, FernandoHess, IsabellO'Meara, Connor P.Schorpp, MichaelBoehm, Thomas2023-05-122023-05-122016-11Cell Reports. Volume 17, Issue 9, Pages 2259 - 2270. 22 November 20162211-1247https://repositorio.unab.cl/xmlui/handle/ria/49575Indexación: Scopus.ymphocytes represent basic components of verte brate adaptive immune systems, suggesting the util ity of non-mammalian models to define the molecular basis of their development and differentiation. Our forward genetic screens in zebrafish for recessive mutations affecting early T cell development re vealed several major genetic pathways. The iden tification of lineage-specific transcription factors and specific components of cytokine signaling and DNA replication and/or repair pathways known from studies of immunocompromised mammals pro vided an evolutionary cross-validation of the screen design. Unexpectedly, however, genes encoding proteins required for pre-mRNA processing were en riched in the collection of mutants identified here. In both zebrafish and mice, deficiency of the splice regulator TNPO3 impairs intrathymic T cell differenti ation, illustrating the evolutionarily conserved and cell-type-specific functions of certain pre-mRNA processing factors for T cell development.enT Cell DevelopmentGenetic ScreenImmunodeficiencyZebrafishMousePre-mRNASplicingArtículoAtribution 4.0 International (CC BY 4.0)DOI: 10.1016/j.celrep.2016.11.003