Jiménez, ConstanzaFernández, JavierAroca, MarcelaBordagaray, María JoséPellegrini, ElizabethContador, JavierHernández, MarcelaValenzuela, FernandoFernández, Alejandra2024-05-312024-05-312023-11International Journal of Molecular Sciences Open Access Volume 24, Issue 21 November 2023 Article number 1559216616596https://repositorio.unab.cl/handle/ria/57223Indexación: Scopus.Emerging epidemiological evidence links atopic dermatitis (AD) and periodontitis, although the mechanisms remain unclear. Th2-derived cytokines are key in the development of both diseases, and different gingival crevicular fluid (GCF) profiles among healthy and diseased subjects have been previously reported. This case–control study examined the GCF levels of interleukins (IL)-13, IL-31, and thymic stromal lymphopoietin (TSLP) in 29 subjects with moderate-to-severe AD and 33 controls. All subjects underwent comprehensive clinical and oral evaluations, followed by GCF collection. GCF levels of IL-13, IL-31, and TSLP were assessed using a multiplex-bead immunoassay. Demographic and periodontal parameters were similar among groups (p > 0.05). The GCF levels of IL-31 and TSLP were higher in AD subjects compared to controls (p < 0.05), whereas no significant differences in the GCF levels of IL-13 were noticed (p = 0.377). Moderate-to-severe AD was positively associated with the GCF levels of IL-31 and TSLP, whereas severe periodontitis was negatively associated with IL-31 (p < 0.05). The GCF levels of IL-13 showed no significant associations with either condition (p = 0.689). There was no significant interaction between AD and periodontitis for IL-31 (p < 0.869). These results suggest that AD and periodontitis independently influence the GCF levels of IL-31 in opposing ways, whereas AD alone influences the levels of TSLP.enatopic dermatitisbiomarkersgingival crevicular fluidinterleukin-13interleukin-31interleukinsperiodontitisthymic stromal lymphopoietinArtículoCC BY 4.0 DEED Attribution 4.0 International10.3390/ijms242115592