Manzanares, DaríoAraya-Durán, IngridGallego-Yerga, LauraJátiva, PabloMárquez-Miranda, ValeriaCanan, JonathanJiménez Blanco, José LuisMellet, Carmen OrtizGonzález-Nilo, Fernando DaniloGarcía Fernández, José ManuelCeña, Valentín2024-04-302024-04-302017-07Nanomedicine Volume 12, Issue 13, Pages 1607 - 1621July 20171743-5889https://repositorio.unab.cl/handle/ria/56487Indexación: ScopusAim: To study the structural requirements that a cyclooligosaccharide-based nanoparticle must fulfill to be an efficient siRNA transfection vector. Materials & methods: siRNA protection from degradation by RNAses, transfection efficiency and the thermodynamic parameters of the nanoparticle/siRNA interactions were studied on pairs of amphiphilic molecules using biochemical techniques and molecular dynamics. Results: The lower the siRNA solvent accessible surface area in the presence of the nanoparticle, higher the protection from RNAse-mediated degradation in the corresponding nanocomplex; a moderate nanoparticle/siRNA binding energy value further facilitates reversible complexation and binding to the target cellular mRNA. Conclusion: The use, in advance, of these parameters will provide a useful indication of the potential of a molecular nanoparticle as siRNA transfecting vector. © 2017 Future Medicine Ltd.enendosomal escapemolecular modelingmolecular nanoparticleprotection from RNAsesprotein knock downsiRNA transfectionArtículoCC BY-NC-ND 4.0 DEED Atribución-NoComercial-SinDerivadas 4.0 Internacional10.2217/nnm-2017-0123