Arratia, FelipeFierro, CristopherBlanco, AlejandroFuentes, SebastiánNahuelquen, DanielaMontecino, MartinRojas, AdrianaAguilar, Rodrigo2023-12-052023-12-052023-06Current Issues in Molecular Biology Open Access Volume 45, Issue 6, Pages 4735 - 4748June 202314673037https://repositorio.unab.cl/xmlui/handle/ria/54415Indexación: Scopus.In cancer cells, the long non-coding RNA (lncRNA) MALAT1 has arisen as a key partner for the Polycomb Repressive Complex 2 (PRC2), an epigenetic modifier. However, it is unknown whether this partnership occurs genome-wide at the chromatin level, as most of the studies focus on single genes that are usually repressed. Due to the genomic binding properties of both macromolecules, we wondered whether there are binding sites shared by PRC2 and MALAT1. Using public genome-binding datasets for PRC2 and MALAT1 derived from independent ChIP- and CHART-seq experiments performed with the breast cancer cell line MCF7, we searched for regions containing PRC2 and MALAT1 overlapping peaks. Peak calls for each molecule were performed using MACS2 and then overlapping peaks were identified by bedtools intersect. Using this approach, we identified 1293 genomic sites where PRC2 and MALAT1 concur. Interestingly, 54.75% of those sites are within gene promoter regions ([removed]enChromatinLong non-coding RNAMALAT1Polycomb Repressive Complex 2ArtículoCC BY 4.0 DEED10.3390/cimb45060301