Hantavirus Gn and Gc glycoproteins self-assemble into virus-like particles

Acuña, RodrigoCifuentes-Muñoz, NicolásMárquez, Chantal L.Bulling, ManuelaKlingström, JonasMancini, RobertaLozach, Pierre-YvesTischlera, Nicole D.2023-06-272023-06-272014-02Journal of Virology. Volume 88, Issue 4, Pages 2344 - 2348. February 20141098-5514https://repositorio.unab.cl/xmlui/handle/ria/51092Indexación: Scopus.How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.enHantavirusVirologíaPuumala (PUUV)ANDVHantavirus Gn and Gc glycoproteins self-assemble into virus-like particlesArtículoAtribution 4.0 International (CC BY 4.0)10.1128/JVI.03118-13