Ardiles, A.Barrientos, R.Simirgiotis, M.J.Bórquez, J.Sepúlveda, B.Areche, C.2019-12-062019-12-062018-09Molecules, 23(9), art. no. 2361.1420-3049DOI: 10.3390/molecules23092361http://repositorio.unab.cl/xmlui/handle/ria/11205Indexación: Scopus.Chemicals and CAS Registry Numbers: alcohol, 64-17-5; hydrochloric acid, 7647-01-0; lansoprazole, 103577-45-3; Anti-Ulcer Agents; Benzofurans; Diterpenes, Clerodane; Ethanol; Hydrochloric Acid; Lansoprazole; Plant Extracts; tremetolForty-three metabolites including several methoxylated flavonoids, tremetones, and ent-clerodane diterpenes were accurately identified for the first time in the ethanolic extract of P. quadrangularis by means of hyphenated UHPLC-quadrupole Orbitrap mass spectrometry, and seven isolated compounds were tested regarding gastroprotective activity using the HCl/EtOH-induced lesion model in mice. A new tremetone (compound 6) is reported based on spectroscopic evidence. The isolated clerodanes and tremetones showed gastroprotective activity in a mouse model, evidenced by compound 7 (p-coumaroyloxytremetone), which showed the highest gastroprotective activity (76%), which was higher than the control drug lansoprazole (72%). Our findings revealed that several constituents of this plant have gastroprotective activity, and particularly, p-coumaroyloxytremetone could be considered as a lead molecule to explore new gastroprotective agents. This plant is a rich source of biologically active tremetones and terpenoids which can support the ethnobotanical use of the plant. © 2018 MDPI AG. All rights reserved.enClerodanesEndemic plantsGastric ulcerGastroprotectiveLC-MS/MSTremetonesArtículo