Díaz-García, V.M.Guerrero, S.Díaz-Valdivia, N.Lobos-González, L.Kogan, M.Pérez-Donoso, J.M.Quest, A.F.G.2019-12-172019-12-172018International journal of nanomedicine, 13, pp. 6391-6412.1176-9114DOI: 10.2147/IJN.S165565http://repositorio.unab.cl/xmlui/handle/ria/11518Indexación: Scopus.The authors appreciate the support of Cellular Communication Laboratory, Faculty of Medicine, Universidad de Chile; The BioNanotechnology and Microbiology Laboratory, Faculty of Biological Sciences, Universidad Andres Bello; The Department of Pharmacological and Toxicological Chemistry, Faculty of Chemical and Pharmaceutical Sciences, Universidad de Chile and Fundación Ciencia y Vida, Santiago, Chile. The authors also appreciate the personal support of the Díaz-García family (VMDG) and Erika Elcira Donoso Lopez (JMPD). The study was funded by CONICYT-FONDAP 15130011 (AQ), FONDECYT 1130250, 1170925 (AQ), Anillo ACT 1111 (AQ, JMPD), FONDECYT 1151255 (JMPD), INACH RT-25–16 (JMPD), AFOSR FA9550-15-1-0140 (JMPD), and Basal de FCV PFB-16 (LLG). A post-doctoral CONICYT award 3140463 (SG) and a CONICYT PhD fellowship 21120816 (VMDG) are also acknowledged.Background: Numerous studies have proposed the use of fluorescent semiconductor nanoparticles or quantum dots (QDs) as novel tools to label cells and tumors. However, QD applications are limited by their toxicity in biological systems and little is known about whether QDs affect the capacity of cancer cells to metastasize. Previously, we described the “biomimetic” synthesis of CdTe-QDs (QDs-glutathione [GSH]) with increased biocompatibility and the potential utility in labeling cells. Purpose: In order to determine the feasibility of using QDs-GSH as a tool for tracking tumor cells during early metastasis, we characterized here for the first time, the in vitro and in vivo effects of the incorporation of green or red biomimetic QDs-GSH into B16F10 cells, a syngeneic mouse melanoma line for metastasis assays in C57BL/6 mice. Methods: B16F10 cells were labeled with green or red biomimetic QDs-GSH in the presence or absence of n-acetylcysteine. Then, migration, invasion and proliferation of labeled B16F10 were evaluated in vitro. Finally, the B16F10 cells labeled with red QDs-GSH were used to monitor in vivo lung metastasis at early time points (5 minutes to 24 hours) or after 21 days in C57BL/6 mice. Results: We developed a methodology that allows obtaining QDs-GSH-labeled B16F10 cells (nearly 100% viable labeled cells), which remained viable for at least 5 days and migrated similarly to control cells. However, proliferation, invasion, and the capacity to form metastatic nodules in the lungs were severely attenuated. Fluorescence imaging revealed that distribu-tion/accumulation of QDs-GSH-labeled B16F10 cells could be tracked following injection into C57BL/6 mice (syngeneic preclinical metastasis model) and that these cells preferentially accumulated in the perialveolar area in lungs as early as 5 minutes post-injection. Conclusion: The methodology described here represents a useful alternative for monitoring initial events during tumor cell metastasis. © 2018 Díaz-García et al.enBiomimeticCancerCell trackingInvasionMigrationProliferationArtículo