Sauma, D.Michea, P.Lennon-Dumenil, A.Fierro, A.Morales, J.Rosemblatt, M.Bono, M.2021-06-152021-06-152004-02Scandinavian Journal of Immunology, Volume 59, Issue 2, Pages 183 - 189February 20040300-9475Indexación: Scopus.Dendritic cells (DCs) generated in vitro from bone marrow precursors using granulocyte-macrophage colony-stimulating factor (GM-CSF) secrete interleukin-2 (IL-2) upon activation, an event probably associated to the initiation of adaptive immune responses. Additionally, they produce IL-12, a cytokine related to T-cell polarization. To analyse the effect of IL-4 on DC differentiation and function, we assessed the capacity of murine bone marrow dendritic cells (BMDCs) differentiated with GM-CSF in the presence or absence of IL-4 to produce IL-2 and IL-12 upon lipopolysaccharide (LPS) activation. We found that although IL-4 enhanced DC IL-12p70 production, it strongly impaired IL-2 secretion by BMDCs. This inhibition, which depends on the presence of IL-4 during LPS activation, is DC specific, as IL-4 did not affect IL-2 secretion by T cells. Interestingly, inhibition of DC IL-2 production did not prevent DC priming of T lymphocytes. These results illustrate a new putative role for IL-4 on the regulation of the immune response and should help clarify the controversial reports on the effect of IL-4 on DCs.enNatural Killer CellsCell DifferentiationCoculture TechniquesDendritic CellsFlow CytometryInterleukin-12Interleukin-2Interleukin-4LipopolysaccharidesLymphocyte ActivationArtículoDOI: 10.1111/j.0300-9475.2004.01380.x