Castro-Córdova, PabloMendoza-León, María JoséParedes-Sabja, Daniel2023-12-072023-12-072021-12PLoS ONE Volume 16, Issue 12 December December 2021 Article number e02610811932-6203https://repositorio.unab.cl/xmlui/handle/ria/54486Indexación (Scopus)Interaction of Clostridioides difficile spores with the intestinal mucosa contributes to the persistence and recurrence of the infection. Advanced age is one of the main risk factors for C. difficile infection and recurrence of the disease. However, interaction of C. difficile spores with the intestinal mucosa during aging has not been evaluated. In the present work, using intestinal ligated loop technique in a mouse model, we analyzed C. difficile spore adherence and internalization to the ileum and colonic mucosa during aging. Additionally, we provide visual documentation of the critical steps of the procedure. Consequently, our data suggest that spore internalization in the ileum and colonic mucosa is higher in elderly mice rather than adults or young mice. Also, our data suggest that spore adherence to the ileum and colonic mucosa decreases with aging.enAgingAnimalsAttachment SitesMicrobiologicalBacterial AdhesionClostridioides difficileClostridium InfectionsDisease Models, AnimalFemaleIntestinal MucosaMaleMiceMice Inbred C57BLMicroscopy, ConfocalSpores, BacterialArtículoCC BY 4.0 Deed Atribución 4.0 Internacional10.1371/journal.pone.0261081