Calderón, Paulina F.Morales, Eduardo H.Acuña, Lillian G.Fuentes, Danitza N.Gil, FernandoPorwollik, S.McClelland, MichaelSaavedra, Claudia P.Calderón, Iván L.2023-06-022023-06-022014-07Biochemical and Biophysical Research Communications. Volume 450, Issue 1, Pages 641 - 645. 18 July 20140006-291Xhttps://repositorio.unab.cl/xmlui/handle/ria/50299Indexación: Scopus.Typically, the expression of sRNAs is activated in response to environmental stimuli in order to regulate gene expression through post-transcriptional mechanisms. In the present work we show that the Salmo nella typhimurium paralogous sRNAs RyhB-1 and RyhB-2 are induced in response to the nitrosating agent S-nitrosoglutathione (GSNO). Inactivation of these sRNAs decreased S. typhimurium resistance to GSNO and increased the levels of nitrosylated proteins. These results prompted us to evaluate a possible role of these sRNAs in nitrosative stress resistance. RNA profiling was used as a screen to identify novel RyhB-1 and RyhB-2 regulated targets. A subset of genes was filtered based on their potential role in the response to nitrosative stress and their expression was analyzed by quantitative RT-PCR in wild type, single and double mutant strains (DryhB1, DryhB2 and DryhB1 DryhB2) treated with GSNO. In response to GSNO RyhB-1 and RyhB-2 negatively regulate the expression of the genes cyoABC (cytochrome bo oxi dase), cydB (cytochrome bd oxidase), cybC (cytochrome b-562), and positively regulate the nirBCD operon (nitrite reductase system). Together, these results suggest that RyhB-1 and RyhB-2 finely tune the expres sion of genes coding for cytochrome oxidases and the nitrate reductase system, allowing the cell to cope with GSNO-induced stress. 2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).ensRNARyhBNitrosative StressGSNORNSArtículoAtribution 4.0 International (CC BY 4.0)10.1016/j.bbrc.2014.06.031