Marzol, ElianaBorassi, CeciliaCarignani Sardoy, MarianaRanocha, PhilippeAptekmann, Ariel A.Bringas, MauroPennington, JanicePaez-Valencia, JulioMartínez Pacheco, JavierRodríguez-Garcia, Diana R.Rondón Guerrero, Yossmayer Del CarmenPeralta, Juan ManuelFleming, MargaretMishler-Elmore, John W.Mangano, SilvinaBlanco-Herrera, FranciscaBedinger, Patricia A.Dunand, ChristopheCapece, LucianaNadra, Alejandro D.Held, MichaelOtegui, Marisa S.Estevez, José M.2024-05-142024-05-142022-05-02International Journal of Molecular Sciences, Volume 23, Issue 10, May-2 2022, Article number 53751661-6596https://repositorio.unab.cl/handle/ria/56813Indexación: Scopus.Root hair cells are important sensors of soil conditions. They grow towards and absorb water-soluble nutrients. This fast and oscillatory growth is mediated by continuous remodeling of the cell wall. Root hair cell walls contain polysaccharides and hydroxyproline-rich glycoproteins, including extensins (EXTs). Class-III peroxidases (PRXs) are secreted into the apoplastic space and are thought to trigger either cell wall loosening or polymerization of cell wall components, such as Tyr-mediated assembly of EXT networks (EXT-PRXs). The precise role of these EXT-PRXs is unknown. Using genetic, biochemical, and modeling approaches, we identified and characterized three root-hair-specific putative EXT-PRXs, PRX01, PRX44, and PRX73. prx01,44,73 triple mutation and PRX44 and PRX73 overexpression had opposite effects on root hair growth, peroxidase activity, and ROS production, with a clear impact on cell wall thickness. We use an EXT fluorescent reporter with contrasting levels of cell wall insolubilization in prx01,44,73 and PRX44-overexpressing background plants. In this study, we propose that PRX01, PRX44, and PRX73 control EXT-mediated cell wall properties during polar expansion of root hair cells. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.enArabidopsisCell wallsClass-III peroxidasesExtensinsRoot hairsROSArtículoCC BY 4.0 DEED Atribución 4.0 Internacional10.3390/ijms23105375