Ruiz, PaulinaMaldonado, PaulaHidalgo, YessiaSauma, DanielaRosemblatt, MarioBono, Maria Rosa2023-04-202023-04-202015-11Frontiers in Immunology Volume 6, Issue NOV2015 Article number 5961664-3224https://repositorio.unab.cl/xmlui/handle/ria/48835Indexación: ScopusThe induction of donor-specific transplant tolerance is one of the main goals of modern immunology. Establishment of a mixed chimerism state in the transplant recipient has proven to be a suitable strategy for the induction of long-term allograft tolerance; however, current experimental recipient preconditioning protocols have many side effects, and are not feasible for use in future therapies. In order to improve the current mixed chimerism induction protocols, we developed a non-myeloablative bone-marrow transplant (NM-BMT) protocol using retinoic acid (RA)-induced alloantigen-specific Tregs, clinically available immunosuppressive drugs, and lower doses of irradiation. We demonstrate that RA-induced alloantigen-specific Tregs in addition to a NM-BMT protocol generates stable mixed chimerism and induces tolerance to allogeneic secondary skin allografts in mice. Therefore, the establishment of mixed chimerism through the use of donor-specific Tregs rather than non-specific immunosuppression could have a potential use in organ transplantation. © 2015 Ruiz, Maldonado, Hidalgo, Sauma, Rosemblatt and Bono.enAlloreactive regulatory T cellsMixed chimerismNon-myeloablative conditioningOrgan transplantationTransplant toleranceArtículoAtribución 4.0 Internacional (CC BY 4.0)10.3389/fimmu.2015.00596