EvaluaciĆ³n del complejo mayor de histocompatibilidad como un marcador de resistencia al IPNv
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2007
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es
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Universidad AndrƩs Bello
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Licencia CC
Resumen
La industria del salmĆ³n, comenzĆ³ en Chile a raĆz de programas cooperativos internacionales entre los gobiernos de Chile y JapĆ³n, e iniciativas privadas. AsĆ se creĆ³ la primera empresa exportadora de salmĆ³n llamada Sociedad de PesquerĆas Lago Llanquihue. Gracias a las excelentes condiciones geogrĆ”ficas que presenta nuestro paĆs, tales como: condiciones de aguas Ć³ptimas, puras y limpias, ademĆ”s de, temperaturas adecuadas, han permitido que Chile presente ventajas competitivas en la industria salmonĆcola mundial (Salgado, 2005). En el aƱo 1986 la industria de la salmonicultura presenta un aumento explosivo, basado fundamentalmente en indicadores de proyectos de factibilidad econĆ³mica que demostraban cifras impresionantes en relaciĆ³n con las tasas internas de retorno ante una eventual exportaciĆ³n del producto. El auge que ha vivido la industria queda de manifiesto con los incrementos que ha obtenido en los retornos de las exportaciones, de US $ 349 millones en 1994 a US $ 1.900 millones en el 2006 (AsociaciĆ³n de la Industria del salmĆ³n Chile). El dinamismo que presenta la industria chilena, ha significado que la participaciĆ³n en la producciĆ³n mundial de salmones en 1994 haya aumentado un 18% y en a un 38,1% en el 2004, (Fig.1 ), superando a Noruega, que ha sido el primer exportador de salmones del mundo durante decenas de aƱos.
Major histocompatibility complex (MHC) genes are involved in the primary recognition of foreing phatogens, activating the inmune response in fish in do a consequence. The identifications of genotypes related of broodstock related to IPNv resistance in fish, during selection of broodstock is presented as a strategy to prevent mortalities due to this viral pathogens. In this research the alelic variability of MHC was assesed in order to find a relation with resistance to IPNv. Three salmonid species were studied: Salmo salar, Oncorhynchus mykiss and Oncorhynchus kisutch. In order to fulfill the objetives (goals) of this work; PCR technique was implemented and optimized to amplify MHC loci. Results were obsrved performing DGGE, and analized by Fisher's exact test. There were no significative differences between amplified loci from surviving fish an dead fish (p< 0.05), This estudy shows the frrst approach in order to develop future research related to MHC alelic variability in salmonid fish.
Major histocompatibility complex (MHC) genes are involved in the primary recognition of foreing phatogens, activating the inmune response in fish in do a consequence. The identifications of genotypes related of broodstock related to IPNv resistance in fish, during selection of broodstock is presented as a strategy to prevent mortalities due to this viral pathogens. In this research the alelic variability of MHC was assesed in order to find a relation with resistance to IPNv. Three salmonid species were studied: Salmo salar, Oncorhynchus mykiss and Oncorhynchus kisutch. In order to fulfill the objetives (goals) of this work; PCR technique was implemented and optimized to amplify MHC loci. Results were obsrved performing DGGE, and analized by Fisher's exact test. There were no significative differences between amplified loci from surviving fish an dead fish (p< 0.05), This estudy shows the frrst approach in order to develop future research related to MHC alelic variability in salmonid fish.
Notas
Tesis (BiĆ³logo Marino, MagĆster en BiologĆa Marina)
Palabras clave
Pancreatitis Aguda Necrotizante, Histocompatibilidad, Industria del SalmĆ³n, Chile