Estudio descriptivo de la microflora presente en los alvéolos con cuadro de alvelitis, antes, durante y después del tratamiento
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Archivos
Fecha
2011
Autores
Profesor/a Guía
Facultad/escuela
Idioma
es
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Universidad Andrés Bello
Nombre de Curso
Licencia CC
Licencia CC
Resumen
Objetivo. Aislar e identificar patógenos presentes en pacientes con Alveolitis seca
antes y después del tratamiento con gasa yodoformada.
Método. Se tomaron 3 muestras con tórula de algodón de la cavidad alveolar de tres
pacientes de la Clínica de Odontología de la Universidad Andrés Bello que presentaban
Alveolitis. La muestra n°1 se tomó previo al tratamiento, la muestra n°2 después de
irrigación con suero fisiológico y la muestra n°3 posterior a un tratamiento por una
semana con gasa yodoformada. Cada muestra fue sembrada en medios sólidos (agar
sangre y agar Mac Conkey) con técnica de aislamiento y se realizó el reaislamiento de
las colonias obtenidas para una posterior caracterización macroscópica y microscópica
con tinción de Gram. Para la identificación se realizaron pruebas bioquímicas clásicas
para las bacterias Gram positivo (catalasa-coagulasa) y la batería bioquímica Api20E
para las bacterias Gram negativo. Finalmente, a los patógenos identificados se les
realizó un ensayo de susceptibilidad a antibióticos.
Resultados. Los organismos que se encontraron fueron 14, repitiéndose en distintas
frecuencias en los distintos pacientes. Los microorganismos con mayor frecuencia de
repetición fueron tres, en primer lugar con el 44% de las muestras fue Streptococcus viridans, en segundo lugar lo hizo Staphylococcus aureus con un 18% de frecuencia y
en tercer lugar con un 10% fue Staphylococcus epidermidis. Conclusión. Las
cavidades alveolares con Alveolitis analizadas presentan bacterias patógenas
oportunistas, lo que sugiere que existe un factor microbiológico asociado a esta
manifestación clínica.
Objective. lsolate and identify pathogens in patients with dry Alveolitis before and after the treatment with iodoform gauze. Methods. 3 samples were taken with a cotton swab to the socket of three patients of the Clinic of Dentistry, Universidad Andres Bello who had Alveolitis. The samples n°1 was taken prior to treatment, the sample n°2 after irrigation with saline and the sample n°3 after treatment for a week with iodoform gauze. Each sample was grown on solid media (blood agar and MacConkey agar) with isolation technique and performed the reisolation of these colonies for further macroscopic and microscopic characterization by Gram stain. To identify classical biochemical tests were performed for Gram-positive bacteria (catalase, coagulase) and the api20E kit test for Gram negative. Finally, the pathogens identified were evaluated by a test of susceptibility to antibiotics. Results. 14 organisms were found, repeating in different sequences on patients. The most repetition frequent microorganism was three, in the first place with the 44% of the samples were Streptococcus viridans, secondly Staphylococcus aureus with 18% of frequency and in third place, Staphylococcus epidermis with 10%. Conclusion. Alveolar socket analyzed present opportunistic pathogenic bacteria, suggesting that there is a microbiological factor associated with this clinical manifestation.
Objective. lsolate and identify pathogens in patients with dry Alveolitis before and after the treatment with iodoform gauze. Methods. 3 samples were taken with a cotton swab to the socket of three patients of the Clinic of Dentistry, Universidad Andres Bello who had Alveolitis. The samples n°1 was taken prior to treatment, the sample n°2 after irrigation with saline and the sample n°3 after treatment for a week with iodoform gauze. Each sample was grown on solid media (blood agar and MacConkey agar) with isolation technique and performed the reisolation of these colonies for further macroscopic and microscopic characterization by Gram stain. To identify classical biochemical tests were performed for Gram-positive bacteria (catalase, coagulase) and the api20E kit test for Gram negative. Finally, the pathogens identified were evaluated by a test of susceptibility to antibiotics. Results. 14 organisms were found, repeating in different sequences on patients. The most repetition frequent microorganism was three, in the first place with the 44% of the samples were Streptococcus viridans, secondly Staphylococcus aureus with 18% of frequency and in third place, Staphylococcus epidermis with 10%. Conclusion. Alveolar socket analyzed present opportunistic pathogenic bacteria, suggesting that there is a microbiological factor associated with this clinical manifestation.
Notas
Tesis (Cirujano Dentista, Especialización en Cirugía)
Palabras clave
Salud Oral, Bacterias, Chile, Alveolitis