Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection
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Fecha
2021-12
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
Nature Research
Nombre de Curso
Licencia CC
Licencia CC
Resumen
Clostridioides difficile spores produced during infection are important for the recurrence of the disease. Here, we show that C. difficile spores gain entry into the intestinal mucosa via pathways dependent on host fibronectin-α5β1 and vitronectin-αvβ1. The exosporium protein BclA3, on the spore surface, is required for both entry pathways. Deletion of the bclA3 gene in C. difficile, or pharmacological inhibition of endocytosis using nystatin, leads to reduced entry into the intestinal mucosa and reduced recurrence of the disease in a mouse model. Our findings indicate that C. difficile spore entry into the intestinal barrier can contribute to spore persistence and infection recurrence, and suggest potential avenues for new therapies. © 2021, The Author(s).
Notas
Indexación Scopus
Palabras clave
Peptoclostridium Difficile, Clostridium Infections, Pseudomembranous Enterocolitis
Citación
Nature Communications Volume 12, Issue 1, December 2021, Article number 1140
DOI
10.1038/s41467-021-21355-5