• Login
    View Item 
    •   DSpace Home
    • INVESTIGACIÓN ACADÉMICA
    • Facultad de Ciencias de la Vida
    • Fac.CV - Artículos de Revista
    • View Item
    •   DSpace Home
    • INVESTIGACIÓN ACADÉMICA
    • Facultad de Ciencias de la Vida
    • Fac.CV - Artículos de Revista
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Lactoferrin Decreases the Intestinal Inflammation Triggered by a Soybean Meal-Based Diet in Zebrafish

    Thumbnail
    View/Open
    TEXTO COMPLETO (3.511Mb)
    Date
    2016
    Author
    Ulloa, Pilar E.
    Solís, Camila J.
    De la Paz, Javiera F.
    Alaurent, Trevor G. S.
    Caruffo, Mario
    Hernández, Adrián J.
    Dantagnan, Patricio
    Feijóo, Carmen G.
    Metadata
    Show full item record
    Abstract
    Intestinal inflammation is a harmful condition in fish that can be triggered by the ingestion of soybean meal. Due to the positive costs-benefits ratio of including soybean meal in farmed fish diets, identifying additives with intestinal anti-inflammatory effects could contribute to solving the issues caused by this plant protein. This study evaluated the effect of incorporating lactoferrin (LF) into a soybean meal-based diet on intestinal inflammation in zebrafish. Larvae were fed with diets containing 50% soybean meal (50SBM) or 50SBM supplemented with LF to 0.5, 1, 1.5 g/kg (50SBM+LF0.5; 50SBM+LF1.0; 50SBM+LF1.5). The 50SBM+LF1.5 diet was the most efficient and larvae had a reduced number of neutrophils in the intestine compared with 50SBM larvae and an indistinguishable number compared with control larvae. Likewise, the transcription of genes involved in neutrophil migration and intestinal mucosal barrier functions (mmp9, muc2.2, and beta-def-1) were increased in 50SBM larvae but were normally expressed in 50SBM+LF1.5 larvae. To determine the influence of intestinal inflammation on the general immune response, larvae were challenged with Edwardsiella tarda. Larvae with intestinal inflammation had increased mortality rate compared to control larvae. Importantly, 50SBM+LF1.5 larvae had a mortality rate lower than control larvae. These results demonstrate that LF displays a dual effect in zebrafish, acting as an intestinal anti-inflammatory agent and improving performance against bacterial infection.
    URI
    http://repositorio.unab.cl/xmlui/handle/ria/827
    Collections
    • Fac.CV - Artículos de Revista

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Documentos Trabajos de TitulaciónProcedimientos de publicaciónDerecho de autorGuía para Trabajos de Titulación pre y post gradoFormulario de AutorizaciónLogo Institucional

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV