Evaluación de las alteraciones hematológicas y de bioquímica clínica asociados al tratamiento con Imatinib en cáncer prostático transgénico
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Archivos
Fecha
2014
Profesor/a Guía
Facultad/escuela
Idioma
es
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Título del volumen
Editor
Universidad Andrés Bello
Nombre de Curso
Licencia CC
Licencia CC
Resumen
La próstata humana es un órgano retroperitoneal que rodea el cuello vesical y la uretra, está provista de una cápsula delimitada [l]. Histológicamente, es una glándula exocrina túbulo alveolar compuesta por tejido glandular rodeado por estroma fibromuscular. Existen diferencias histológicas en distintas especies, en humanos la próstata presenta sub regiones que no están bien definidas y de una alta complejidad, entre ellas están: zona central, zona de transición y la zona periférica, la cual contiene el tejido glandular que corresponde al sitio más frecuente de origen de cáncer. En ratones, la próstata normal es una glándula dividida en cuatro lóbulos
bien definidos: dorsal, lateral, ventral y anterior, los cuales difieren histológicarnente uno de otro [3], es por esta razón que el modelo murino TRAMP (adenocarcinoma prostático de ratón transgénico), es un modelo que provee de mucha información referente a los estadios del cáncer prostático humano.
La patogenia del cáncer prostático es multifactorial y se ha establecido que para que haya un crecimiento tumoral se requieren factores genéticos que participen directamente, favoreciendo el crecimiento y proliferación, asociándose a células inflamatorias leucocitarias como macrófagos, neutrófilos y mastocitos.
La investigación consiste en probar la eficacia del fármaco Imanitib (Masivet@) a través de análisis micrométricos de variables hematológicas y de bioquímica clínica para estudiar el cáncer de próstata, utilizando ratones manipulados genéticamente (transgénicos), observando las variaciones que ocurrirán en un tiempo determinado. Además, se pretende estandarizar el método de extracción de la muestra y determinar el volumen mínimo que se requiere para poder medir todos los parámetros incluidos en ambos exámenes.
The human prostate it is a retroperitoneal organ around the bladder neck and urethra, it is provided with a defined capsule [1]. Histologically, it is an exocrine tubule alveolar gland composed of glandular tissue surrounded by stroma muscle fiber. Histological differences exist in different species, in human the prostate has sub regions that are not well defined and highly complex, among them are: central zone, transition zone and peripheral zone, which contains the glandular tissue that corresponds to the site most frequent origin of cancer. [2] In mice, the normal prostate gland is divided into four distinct lobes: dorsal, lateral, ventral and anterior, which histologically differ from each other [3], it is for this reason that the murine model TRAMP (prostatic adenocarcinoma of transgenic mouse ) it is a model that provides a lot of information regarding the stages of human prostate cancer. The prostate cancer pathogenesis is multifactorial and it has been established that for tumor growth, genetic factors direct involved are required, favoring the growth and proliferation, associated with leukocyte inflammatory cells such as macrophages, neutrophils and mast cells. [1] The research consist to test the through micrometric analysis of clinical biochemistry to study prostate drug's effectiveness Imanitib (Masivet®) hematological variables and cancer using m ice genetically engineered (transgenic), watching the variations that will occur in a determínate time. Another aim is to standardize the method of extraction of the sample and determine the mínimum volume required to measure all the parameters included in both tests.
The human prostate it is a retroperitoneal organ around the bladder neck and urethra, it is provided with a defined capsule [1]. Histologically, it is an exocrine tubule alveolar gland composed of glandular tissue surrounded by stroma muscle fiber. Histological differences exist in different species, in human the prostate has sub regions that are not well defined and highly complex, among them are: central zone, transition zone and peripheral zone, which contains the glandular tissue that corresponds to the site most frequent origin of cancer. [2] In mice, the normal prostate gland is divided into four distinct lobes: dorsal, lateral, ventral and anterior, which histologically differ from each other [3], it is for this reason that the murine model TRAMP (prostatic adenocarcinoma of transgenic mouse ) it is a model that provides a lot of information regarding the stages of human prostate cancer. The prostate cancer pathogenesis is multifactorial and it has been established that for tumor growth, genetic factors direct involved are required, favoring the growth and proliferation, associated with leukocyte inflammatory cells such as macrophages, neutrophils and mast cells. [1] The research consist to test the through micrometric analysis of clinical biochemistry to study prostate drug's effectiveness Imanitib (Masivet®) hematological variables and cancer using m ice genetically engineered (transgenic), watching the variations that will occur in a determínate time. Another aim is to standardize the method of extraction of the sample and determine the mínimum volume required to measure all the parameters included in both tests.
Notas
Tesis (Tecnólogo Médico, Especialidad en Bioanálisis Clínico Inmunohematología y Banco de Sangre)
Palabras clave
Cáncer de Próstata, Terapia, Medicamentos, Efectos