Examinando por Autor "Arriagada, C."
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Ítem Cell wall calcium and hemicellulose have a role in the fruit firmness during storage of blueberry (Vaccinium spp.)(MDPI AG, 2021-03) Olmedo, P.; Rojas, B.; Silva-Sanzana, C.; Delgado-Rioseco, J.; Fernández, K.; Balic, I.; Arriagada, C.; Moreno, A.A.; Defilippi, B.G.; Campos-Vargas, R.The firmness of blueberry is one of its most significant quality attributes. Modifications in the composition of the cell wall have been associated with changes in the fruit firmness. In this work, cell wall components and calcium concentration in two blueberry cultivars with contrasting firmness phenotypes were evaluated at harvest and 30 days cold storage (0◦ C). High performance anion-exchange chromatography with pulse amperometric detector (HPAEC-PAD) analysis was performed using the “Emerald” (firmer) and “Jewel” (softer) blueberry cultivars, showing increased glucose in the firmer cultivar after cold storage. Moreover, the LM15 antibody, which recognizes xyloglucan domains, displayed an increased signal in the Emerald cultivar after 30 d cold storage. Additionally, the antibody 2F4, recognizing a homogalacturonan calcium-binding domain, showed a greater signal in the firmer Emerald blueberries, which correlates with a higher calcium concentration in the cell wall. These findings suggest that xyloglucan metabolism and a higher concentration of cell wall calcium influenced the firmness of the blueberry fruit. These results open new perspectives regarding the role of cell wall components as xyloglucans and calcium in blueberry firmness. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Ítem Late Cretaceous–early Eocene counterclockwise rotation of the Fueguian Andes and evolution of the Patagonia–Antarctic Peninsula system(Elsevier B.V., 2016-02) Poblete, F.; Roperch, P.; Arriagada, C.; Ruffet, G.; Ramírez de Arellano, C.; Hervé, F.; Poujol, M.The southernmost Andes of Patagonia and Tierra del Fuego present a prominent arc-shaped structure: the Patagonian Bend. Whether the bending is a primary curvature or an orocline is still matter of controversy. New pa leomagnetic data have been obtained south of the Beagle Channel in 39 out of 61 sites. They have been drilled in Late Jurassic and Early Cretaceous sediments and interbedded volcanics and in mid-Cretaceous to Eocene intrusives of the Fuegian Batholith. The anisotropy of magnetic susceptibility was measured at each site and the influence of mag netic fabric on the characteristic remanent magnetizations (ChRM) in plutonic rocks was corrected using inverse tensors of anisotropy of remanent magnetizations. Normal polarity secondary magnetizations with west-directed declination were obtained in the sediments and they did not pass the fold test. These characteristic directions are similar to those recorded by mid Cretaceous intrusives suggesting a remagnetization event during the normal Cre taceous superchron and describe a large (N90°) counterclockwise rotation. Late Cretaceous to Eocene rocks of the Fueguian Batholith, record decreasing counterclockwise rotations of 45° to 30°. These paleomagnetic results are interpreted as evidence of a large counterclockwise rotation of the Fueguian Andes related to the closure of the Rocas Verdes Basin and the formation of the Darwin Cordillera during the Late Cretaceous and Paleocene. The tectonic evolution of the Patagonian Bend can thus be described as the formation of a progressive arc from an oroclinal stage during the closure of the Rocas Verdes basin to a mainly primary arc during the final stages of deformation of the Magallanes fold and thrust belt. Plate reconstructions show that the Antarctic Peninsula would have formed a continuous margin with Patagonia between the Early Cretaceous and the Eocene, and acted as a non-rotational rigid block facilitating the development of the Patagonian Bend. © 2015 Elsevier B.V. All rights reserved.Ítem The curved Magallanes fold and thrust belt: tectonic insights from a paleomagnetic and anisotropy of magnetic susceptibility study(Blackwell Publishing Ltd, 2014-12) Poblete, F.; Roperch, P.; Hervé, F.; Diraison, M.; Espinoza, M.; Arriagada, C.The Magallanes fold and thrust belt (FTB) presents a large-scale curvature from N-S oriented structures north of 52°S to nearly E-W in Tierra del Fuego Island. We present a paleomagnetic and anisotropy of magnetic susceptibility (AMS) study from 85 sites sampled in Cretaceous to Miocene marine sediments. Magnetic susceptibility is lower than 0.0005 SI for 76 sites and mainly controlled by paramagnetic minerals. AMS results indicate that the sedimentary fabric is preserved in the undeformed areas of Tierra del Fuego and the more external thrust sheets units, where an incipient lineation due to layer parallel shortening is recorded. Prolate AMS ellipsoids, indicating a significant tectonic imprint in the AMS fabric, are observed in the internal units of the belt. AMS results show a good correlation between the orientation of the magnetic lineation and the fold axes. However, in Península Brunswick, the AMS lineations are at ~20° counterclockwise to the strike of the fold axes. Pretectonic stable characteristic remanent magnetizations (ChRM) were determined in seven sites. A counterclockwise rotation (21.2° ± 9.2°) is documented by ChRM data from four sites near the hinge of the belt in Península Brunswick and near Canal Whiteside while there is no evidence of rotation near the nearly E-W oriented Vicuña thrust within Tierra del Fuego. The curved shape of the Cenozoic Magallanes FTB is not related to vertical axis rotation, and thus, the Magallanes FTB can be considered as a primary arc.Ítem Transcriptome analysis during ripening of table grape berry cv. Thompson Seedless(Public Library of Science, 2018) Balic, I.; Vizoso, P.; Nilo-Poyanco, R.; Sanhueza, D.; Olmedo, P.; Sepúlveda, P.; Arriagada, C.; Defilippi, B.G.; Meneses, C.; Campos-Vargas, R.Ripening is one of the key processes associated with the development of major organoleptic characteristics of the fruit. This process has been extensively characterized in climacteric fruit, in contrast with non-climacteric fruit such as grape, where the process is less understood. With the aim of studying changes in gene expression during ripening of non-climacteric fruit, an Illumina based RNA-Seq transcriptome analysis was performed on four developmental stages, between veraison and harvest, on table grapes berries cv Thompson Seedless. Functional analysis showed a transcriptional increase in genes related with degradation processes of chlorophyll, lipids, macromolecules recycling and nucleosomes organization; accompanied by a decrease in genes related with chloroplasts integrity and amino acid synthesis pathways. It was possible to identify several processes described during leaf senescence, particularly close to harvest. Before this point, the results suggest a high transcriptional activity associated with the regulation of gene expression, cytoskeletal organization and cell wall metabolism, which can be related to growth of berries and firmness loss characteristic to this stage of development. This high metabolic activity could be associated with an increase in the transcription of genes related with glycolysis and respiration, unexpected for a non-climacteric fruit ripening.