Examinando por Autor "Blanco-Herrera, Francisca"
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Ítem A Recipe for Success: Three Key Strategies Used by Aphids and Pseudomonas syringae to Colonize the Phyllosphere(Springer, 2023-01) Silva-Sanzana, Christian; Gangas, Maria Victoria; Zavala, Diego; Blanco-Herrera, FranciscaAphids and Pseudomonas syringae are a permanent challenge for agriculture, causing severe losses to the crop industry worldwide. Despite the obvious phylogenetic distance between them, both have become predominant colonizers of the plant kingdom. In this study, we reviewed three key steps of spread and colonization that aphids and P. syringae have mastered to successfully colonize the phyllosphere. These steps involve (i) plant-to-plant movement for locating new nutritional sources, (ii) disruption and modification of the apoplast to facilitate nutrient acquisition, and (iii) suppression of host defenses through effector proteins. In addition, we will provide insights about the direct interaction between aphids and P. syringae and how this yet underrated phenomenon could bring new ecological implications for both organisms beyond their pathogenicity. © 2022, The Author(s).Ítem Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana(MDPI, 2022-05-02) Marzol, Eliana; Borassi, Cecilia; Carignani Sardoy, Mariana; Ranocha, Philippe; Aptekmann, Ariel A.; Bringas, Mauro; Pennington, Janice; Paez-Valencia, Julio; Martínez Pacheco, Javier; Rodríguez-Garcia, Diana R.; Rondón Guerrero, Yossmayer Del Carmen; Peralta, Juan Manuel; Fleming, Margaret; Mishler-Elmore, John W.; Mangano, Silvina; Blanco-Herrera, Francisca; Bedinger, Patricia A.; Dunand, Christophe; Capece, Luciana; Nadra, Alejandro D.; Held, Michael; Otegui, Marisa S.; Estevez, José M.Root hair cells are important sensors of soil conditions. They grow towards and absorb water-soluble nutrients. This fast and oscillatory growth is mediated by continuous remodeling of the cell wall. Root hair cell walls contain polysaccharides and hydroxyproline-rich glycoproteins, including extensins (EXTs). Class-III peroxidases (PRXs) are secreted into the apoplastic space and are thought to trigger either cell wall loosening or polymerization of cell wall components, such as Tyr-mediated assembly of EXT networks (EXT-PRXs). The precise role of these EXT-PRXs is unknown. Using genetic, biochemical, and modeling approaches, we identified and characterized three root-hair-specific putative EXT-PRXs, PRX01, PRX44, and PRX73. prx01,44,73 triple mutation and PRX44 and PRX73 overexpression had opposite effects on root hair growth, peroxidase activity, and ROS production, with a clear impact on cell wall thickness. We use an EXT fluorescent reporter with contrasting levels of cell wall insolubilization in prx01,44,73 and PRX44-overexpressing background plants. In this study, we propose that PRX01, PRX44, and PRX73 control EXT-mediated cell wall properties during polar expansion of root hair cells. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Ítem Draft Genome Sequence of Pseudomonas syringae RAYR-BL, a Strain Isolated from Natural Accessions of Arabidopsis thaliana Plants(American Society for Microbiology, 2022-01) Fuenzalida-Valdivia, Isabel; Gangas, Maria Victoria; Zavala, Diego; Herrera-Vásquez, Ariel; Roux, Fabrice; Meneses, Claudio; Blanco-Herrera, FranciscaHere, we report the genome sequence of the P. syringae strain RAYR-BL, isolated from natural accessions of Arabidopsis plants. The draft genome sequence consists of 5.85 Mbp assembled in 110 contigs. The study of P. syringae RAYR-BL is a valuable tool to investigate molecular features of plant-pathogen interaction under environmental conditions. Copyright © 2022 Fuenzalida-Valdivia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.Ítem Identification of grapevine clones via high-throughput amplicon sequencing: a proof-of-concept study(Genetics Society of America, 2023-09) Urra, Claudio; Sanhueza, Dayan; Pavez, Catalina; Tapia, Patricio; Núñez-Lillo, Gerardo; Minio, Andrea; Miossec, Matthieu; Blanco-Herrera, Francisca; Gainza, Felipe; Castro, Alvaro; Cantu, Dario; Meneses, ClaudioWine cultivars are available to growers in multiple clonal selections with agronomic and enological differences. Phenotypic differences between clones originated from somatic mutations that accrued over thousands of asexual propagation cycles. Genetic diversity between grape cultivars remains unexplored, and tools to discriminate unequivocally clones have been lacking. This study aimed to uncover genetic variations among a group of clonal selections of 4 important Vitis vinifera cultivars: Cabernet sauvignon, Sauvignon blanc, Chardonnay, and Merlot, and use this information to develop genetic markers to discriminate the clones of these cultivars. We sequenced with short-read sequencing technology the genomes of 18 clones, including biological replicates for a total of 46 genomes. Sequences were aligned to their respective cultivar’s reference genome for variant calling. We used reference genomes of Cabernet sauvignon, Chardonnay, and Merlot and developed a de novo genome assembly of Sauvignon blanc using long-read sequencing. On average, 4 million variants were detected for each clone, with 74.2% being single nucleotide variants and 25.8% being small insertions or deletions (InDel). The frequency of these variants was consistent across all clones. From these variants, we validated 46 clonal markers using high-throughput amplicon sequencing for 77.7% of the evaluated clones, most of them small InDel. These results represent an advance in grapevine genotyping strategies and will benefit the viticulture industry for the characterization and identification of the plant material. © The Author(s) 2023.Ítem Metabolite Profiling Reveals the Effect of Cold Storage on Primary Metabolism in Nectarine Varieties with Contrasting Mealiness(MDPI, 2023-02) Olmedo, Patricio; Zepeda, Baltasar; Delgado-Rioseco, Joaquín; Leiva, Carol; Moreno, Adrián A.; Sagredo, Karen; Blanco-Herrera, Francisca; Pedreschi, Romina; Infante, Rodrigo; Meneses, Claudio; Campos-Vargas, ReinaldoChilling injury is a physiological disorder caused by cold storage in peaches and nectarines. The main symptom of chilling injury is mealiness/wooliness, described as a lack of juice in fruit flesh. In this work, we studied two nectarine varieties (Andes Nec-2 and Andes Nec-3) with contrasting susceptibility to mealiness after cold storage. A non-targeted metabolomic analysis was conducted by GC-MS to understand if changes in metabolite abundance are associated with nectarine mealiness induced by cold storage. Multivariate analyses indicated that in unripe nectarines, cold storage promoted a higher accumulation of amino acids in both varieties. Interestingly, for ripe nectarines, cold storage induced an accumulation of fewer amino acids in both varieties and showed an increased abundance of sugars and organic acids. A pathway reconstruction of primary metabolism revealed that in ripe nectarines, cold storage disrupted metabolite abundance in sugar metabolism and the TCA cycle, leading to a differential accumulation of amino acids, organic acids, and sugars in mealy and juicy nectarines. © 2023 by the authorsÍtem Oligogalacturonides Enhance Resistance against Aphids through Pattern-Triggered Immunity and Activation of Salicylic Acid Signaling(MDPI, 2022-09) Silva-Sanzana, Christian; Zavala, Diego; Moraga, Felipe; Herrera-Vásquez, Ariel; Blanco-Herrera, FranciscaThe remarkable capacity of the generalist aphid Myzus persicae to resist most classes of pesticides, along with the environmental and human health risks associated with these agrochemicals, has necessitated the development of safer and greener solutions to control this agricultural pest. Oligogalacturonides (OGs) are pectin-derived molecules that can be isolated from fruit industry waste. OGs have been shown to efficiently stimulate plant defenses against pathogens such as Pseudomonas syringae and Botrytis cinerea. However, whether OGs confer resistance against phytophagous insects such as aphids remains unknown. Here, we treated Arabidopsis plants with OGs and recorded their effects on the feeding performance and population of M. persicae aphids. We also identified the defense mechanism triggered by OGs in plants through the analysis of gene expression and histological approaches. We found that OG treatments increased their resistance to M. persicae infestation by reducing the offspring number and feeding performance. Furthermore, this enhanced resistance was related to a substantial accumulation of callose and reactive oxygen species and activation of the salicylic acid signaling pathway. © 2022 by the authors.Ítem Rhamnolipid-Stabilized Essential Oils Nanoemulsions: Sustainable Biopesticides and Biostimulants with Potential for Crop Protection(Multidisciplinary Digital Publishing Institute (MDPI), 0025) Kourdova, Lucille T.; Mottola, Milagro; Peppino Margutti, Micaela; Bogino, María Florencia; Maritano, Paula; Vico, Raquel Viviana; Blanco-Herrera, Francisca; Fanani, María Laura; Fabro, GeorginaThe search for environmentally friendly solutions to effectively control crop pests while safeguarding human health has become a global priority. One promising strategy is to enhance plant defenses by pre-inducing their innate immune system. In this study, we developed rhamnolipid (RL)-stabilized nanoemulsions (NEs) encapsulating essential oils (EOs) as potential biopesticides and biostimulants for agroindustrial applications. These NEs were designed to improve the solubility and stability of EOs while effectively combining their insecticidal and/or repellent activities with the bioactive properties of RLs. In this regard, our interdisciplinary approach involved formulating and characterizing these NEs and evaluating their stability and wettability on plant leaf surfaces. We further evaluated their effects on bacterial growth in vitro and in the model plant Arabidopsis thaliana, along with their impact on beneficial soil microorganisms. We analyzed their ability to stimulate the plant’s immune system and their impact on the viability and reproduction of the aphid Myzus persicae. Additionally, we explored whether RLs stimulate plant defenses through alterations in the leaf cuticle. Our findings demonstrate that RL-stabilized EO-NEs are effective bioprotectants and biostimulants in the model plant, offering a sustainable alternative that could reduce reliance on chemical pesticides in agriculture. © 2025 by the authors.Ítem The Root Hair Specific SYP123 Regulates the Localization of Cell Wall Components and Contributes to Rizhobacterial Priming of Induced Systemic Resistance(FRONTIERS MEDIA SA, 2016-07) Rodriguez-Furlán, Cecilia; Salinas-Grenet, Hernán; Sandoval, Omar; Recabarren, Camilo; Arraño-Salinas, Paulina; Soto-Alvear, Sylvana; Orellana, Ariel; Blanco-Herrera, FranciscaRoot hairs are important for nutrient and water uptake and are also critically involved the interaction with soil inhabiting microbiota. Root hairs are tubular-shaped outgrowths that emerge from trichoblasts. This polarized elongation is maintained and regulated by a robust mechanism involving the endomembrane secretory and endocytic system. Members of the syntaxin family of SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) in plants (SYP), have been implicated in regulation of the fusion of vesicles with the target membranes in both exocytic and endocytic pathways. One member of this family. SYP123, is expressed specifically in the root hairs and accumulated in the growing tip region. This study shows evidence of the SYP123 role in polarized trafficking using knockout insertional mutant plants. We were able to observe defects in the deposition of cell wall proline rich protein PRP3 and cell wall polysaccharides. In a complementary strategy, similar results were obtained using a plant expressing a dominant negative soluble version of SYP123 (SP2 fragment) lacking the transmembrane domain. The evidence presented indicates that SYP123 is also regulating PRP3 protein distribution by recycling by endocytosis. We also present evidence that indicates that SYP123 is necessary for the response of roots to plant growth promoting rhizobacterium (PGPR) in order to trigger trigger induced systemic response (ISR). Plants with a defective SYP123 function were unable to mount a systemic acquired resistance in response to bacterial pathogen infection and ISR upon interaction with rhizobacteria. These results indicated that SYP123 was involved in the polarized localization of protein and polysaccharides in growing root hairs and that this activity also contributed to the establishment of effective plant defense responses. Root hairs represent very plastic structures were many biotic and abiotic factors can affect the number, anatomy and physiology of root hairs. Here, we presented evidence that indicates that interactions with soil PGPR could be closely regulated by signaling involving secretory and/or endocytic trafficking at the root hair tip as a quick way to response to changing environmental conditions.Ítem The UDP-glucose: Glycoprotein glucosyltransferase (UGGT), a key enzyme in ER quality control, plays a significant role in plant growth as well as biotic and abiotic stress in Arabidopsis thaliana(BioMed Central Ltd., 2015-06) Blanco-Herrera, Francisca; Moreno, Adrián A.; Tapia, Rodrigo; Reyes, Francisca; Araya, Macarena; D'Alessio, Cecilia; Parodi, Armando; Orellana, ArielBackground: UDP-glucose: glycoprotein glucosyltransferase (UGGT) is a key player in the quality control mechanism (ER-QC) that newly synthesized glycoproteins undergo in the ER. It has been shown that the UGGT Arabidopsis orthologue is involved in ER-QC; however, its role in plant physiology remains unclear. Results: Here, we show that two mutant alleles in the At1g71220 locus have none or reduced UGGT activity. In wild type plants, the AtUGGT transcript levels increased upon activation of the unfolded protein response (UPR). Interestingly, mutants in AtUGGT exhibited an endogenous up-regulation of genes that are UPR targets. In addition, mutants in AtUGGT showed a 30 % reduction in the incorporation of UDP-Glucose into the ER suggesting that this enzyme drives the uptake of this substrate for the CNX/CRT cycle. Plants deficient in UGGT exhibited a delayed growth rate of the primary root and rosette as well as an alteration in the number of leaves. These mutants are more sensitive to pathogen attack as well as heat, salt, and UPR-inducing stressors. Additionally, the plants showed impairment in the establishment of systemic acquired resistance (SAR). Conclusions: These results show that a lack of UGGT activity alters plant vegetative development and impairs the response to several abiotic and biotic stresses. Moreover, our results uncover an unexpected role of UGGT in the incorporation of UDP-Glucose into the ER lumen in Arabidopsis thaliana. © Blanco-Herrera et al.Ítem Unravelling the molecular regulation mechanisms of slow ripening trait in prunus persica(MDPI, 2021-11) Núñez-Lillo, Gerardo; Ulloa-Zepeda, Lissette; Pavez, Catalina; Riveros, Anibal; Blanco-Herrera, Francisca; Campos-Vargas, Reinaldo; Pedreschi, Romina; Meneses, ClaudioFruit development is a complex process that involves the interplay of cell division, expan-sion, and differentiation. As a model to study fruit development, nectarines incapable of ripening were described as slow ripening. Slow ripening fruits remained firm and exhibited no rise in CO2 or ethylene production rates for one month or more at 20◦ C. Different studies suggest that this trait is controlled by a single gene (NAC072). Transcriptome analysis between normal and slow ripening fruits showed a total of 157, 269, 976, and 5.224 differentially expressed genes in each fruit developmental stage analyzed (T1, T2, T3, and T7, respectively), and no expression of NAC072 was found in the slow ripening individuals. Using this transcriptomic information, we identified a correlation of NAC072 with auxin-related genes and two genes associated with terpene biosynthesis. On the other hand, significant differences were observed in hormonal biosynthetic pathways during fruit development between the normal and slow ripening individuals (gibberellin, ethylene, jasmonic acid and abscisic acid). These results suggest that the absence of NAC072 by the direct or indirect expression or control of auxins or terpene-related genes prevents normal peach fruit development. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.