Examinando por Autor "Carrillo-Garcia, J."

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    Structural determinants of 5′,6′-epoxyeicosatrienoic acid binding to and activation of TRPV4 channel
    (Nature Publishing Group, 2017-12) Berna-Erro, A.; Izquierdo-Serra, M.; Sepúlveda, R.V.; Rubio-Moscardo, F.; Doñate-Macián, P.; Serra, S.A.; Carrillo-Garcia, J.; Perálvarez-Marín, A.; González-Nilo, F.; Fernández-Fernández, J.M.; Valverde, M.A.
    TRPV4 cation channel activation by cytochrome P450-mediated derivatives of arachidonic acid (AA), epoxyeicosatrienoic acids (EETs), constitute a major mechanisms of endothelium-derived vasodilatation. Besides, TRPV4 mechano/osmosensitivity depends on phospholipase A2 (PLA2) activation and subsequent production of AA and EETs. However, the lack of evidence for a direct interaction of EETs with TRPV4 together with claims of EET-independent mechanical activation of TRPV4 has cast doubts on the validity of this mechanism. We now report: 1) The identification of an EET-binding pocket that specifically mediates TRPV4 activation by 5′,6′-EET, AA and hypotonic cell swelling, thereby suggesting that all these stimuli shared a common structural target within the TRPV4 channel; and 2) A structural insight into the gating of TRPV4 by a natural agonist (5′,6′-EET) in which K535 plays a crucial role, as mutant TRPV4-K535A losses binding of and gating by EET, without affecting GSK1016790A, 4α-phorbol 12,13-didecanoate and heat mediated channel activation. Together, our data demonstrates that the mechano- and osmotransducing messenger EET gates TRPV4 by a direct action on a site formed by residues from the S2-S3 linker, S4 and S4-S5 linker.