Examinando por Autor "D. Barrie, Johnson"

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    Acidithiobacillus ferrianus sp. nov.: an ancestral extremely acidophilic and facultatively anaerobic chemolithoautotroph
    (Springer, 2020-03) R. Norris, Paul; Falagán, Carmen; Moya‑Beltrán, Ana; Castro, Matías; Quatrini, Raquel; D. Barrie, Johnson
    Strain MG, isolated from an acidic pond sediment on the island of Milos (Greece), is proposed as a novel species of fer rous iron- and sulfur-oxidizing Acidithiobacillus. Currently, four of the eight validated species of this genus oxidize ferrous iron, and strain MG shares many key characteristics with these four, including the capacities for catalyzing the oxidative dissolution of pyrite and for anaerobic growth via ferric iron respiration. Strain MG also grows aerobically on hydrogen and anaerobically on hydrogen coupled to ferric iron reduction. While the 16S rRNA genes of the iron-oxidizing Acidi thiobacillus species (and strain MG) are located in a distinct phylogenetic clade and are closely related (98–99% 16S rRNA gene identity), genomic relatedness indexes (ANI/dDDH) revealed strong genomic divergence between strain MG and all sequenced type strains of the taxon, and placed MG as the frst cultured representative of an ancestral phylotype of iron oxidizing acidithiobacilli. Strain MG is proposed as a novel species, Acidithiobacillus ferrianus sp. nov. The type strain is MGT (=DSM 107098T=JCM 33084T). Similar strains have been found as isolates or indicated by cloned 16S rRNA genes from several mineral sulfde mine sites.
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    Genomic evolution of the class Acidithiobacillia: deep-branching Proteobacteria living in extreme acidic conditions
    (Springer Nature, 2021-11) Moya-Beltrán, Ana; Beard, Simón; Rojas-Villalobos, Camila; Issotta, Francisco; Gallardo, Yasna; Ulloa, Ricardo; Giaveno, Alejandra; Degli Esposti, Mauro; D. Barrie, Johnson; Quatrini, Raquel
    Members of the genus Acidithiobacillus, now ranked within the class Acidithiobacillia, are model bacteria for the study of chemolithotrophic energy conversion under extreme conditions. Knowledge of the genomic and taxonomic diversity of Acidithiobacillia is still limited. Here, we present a systematic analysis of nearly 100 genomes from the class sampled from a wide range of habitats. Some of these genomes are new and others have been reclassified on the basis of advanced genomic analysis, thus defining 19 Acidithiobacillia lineages ranking at different taxonomic levels. This work provides the most comprehensive classification and pangenomic analysis of this deep-branching class of Proteobacteria to date. The phylogenomic framework obtained illuminates not only the evolutionary past of this lineage, but also the molecular evolution of relevant aerobic respiratory proteins, namely the cytochrome bo3 ubiquinol oxidases. © 2021, The Author(s).