Examinando por Autor "ESCOBAR, CARLOS A."

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    Ítem
    ELECTROCHEMICAL CHARACTERIZATION OF NEW 1,5-BENZODIAZEPINE DERIVATIVES
    (Sociedad Chilena de Química, 2014-07) JARA-ULLOA, PAOLA; CATALÁN-CARO, SEBASTIÁN; ESCOBAR, CARLOS A.
    Cyclic voltammetry was used to study the electrochemical behavior of new 1,5-benzodiazepine derivatives on a glassy carbon electrode. Well-defined oxidation peaks were observed in DMSO. The Electrochemical response of the glassy carbon electrode was evaluated as function of the scan rate, showing that the electron transfer process for compound (5) and (6) resulted to be controlled by diffusion.
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    Ítem
    SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY
    (Sociedad Chilena de Química, 2014-07) ARAYA, EYLEEN; KOGAN, MARCELO J.; GUELL, ALEIX G.; ESCOBAR, CARLOS A.; SANZ, FAUSTO
    A hydrogen-terminated Si (111) surface was modified to form an aminoterminated monolayer for immobilization of streptavidin. Cleavage of an N-(ω-undecylenyl)-phthalimide covered surface using hidrazine yields an amino group-modified surface, which serves as a substrate for the attachment of biotin and subsequently streptavidin. We used surface analytical techniques to characterize the surface and to control the course of functionalization before the immobilization of streptavidin. To confirm the presence of the streptavidin Texas red on the surface two powerful techniques available in a standard biochemical laboratory are used, Fluorescence Microscopy and MALDI-TOF that allow us to detect and determine the immobilized streptavidin. This work provides an avenue for the development of devices in which the exquisite binding specificity of biomolecular recognition is directly coupled to a biosensor. In addition, we have demonstrated that MALDI-TOF and fluorescence microscopy are useful techniques for the characterization of silicon functionalized surfaces.
  • Miniatura
    Ítem
    SYNTESIS AND CRYSTALLINE STRUCTURE OF THE EXO-3,6-DIMETHYL-3,6-EPOXI-1,2,3,6-TETRAHYDROPHTLALIMIDE AND ITS N-BROMODECYL ANALOG: TWO THERMALLY LABILE DIELS-ALDER ADDUCTS
    (Sociedad Chilena de Química, 2014-07) VÁSQUEZ, RODRIGO; VETTERLEIN, CLAUDIA; TRUJILLO, ALEXANDER; ESCOBAR, CARLOS A.; ARAYA-FUENTES, EYLEEN
    The molecular structure of the exo-3,6-dimethyl-3,6-epoxi-1,2,3,6-tetrahydrophthalimide (1), determined by X-ray diffraction analysis, as well as, its complete spectroscopic characterization and the synthesis and complete spectroscopic characterization of its N-(10-bromodecyl) analog (2) are presented.